摘要
目的探讨HLA-DR和HLA-DQ单倍型在深圳地区结核病发病机制中的作用及机制。方法采用PCR-SSP技术对146例深圳地区肺结核患者及146例健康志愿者的HLA-DR的31个等位基因和HLA-DQ基因的8个等位基因行分型,比较两组间各等位基因频率(GF)和单倍型频率(HF)并计算其优势比(OR)。结果肺结核组DRB1*1601-1605/1607-1608、DRB1*040101-44位点的基因频率显著高于对照组(P均<0.05),其GF比和OR分别为14.39%vs8.59%、19.33%vs10.86%、1.85vs2.08;DR4-DRB4和DR16-DRB5单倍型频率在两组人群间有显著差异,肺结核组明显高于对照组(P均<0.05),其OR分别为2.35,2.97。结论DR4-DRB4和DR16-DRB5单倍型频率在两组人群间的显著差异可能分别是由DRB1*040101-44(DR4)和DRB1*1601-1605/1607-1608(DR16)在两组间的显著差异造成,后两者可能是深圳地区结核病发病的易感基因。
Objective To investigate possible role of HLA-DR and HLA-DQ genotype and haplotype in incidence of pulmonary tuberculosis in Han nationality of Shenzhen in China. Methods C, enotype frequencies and haplotype frequencies of HLA-DR and HLA-DQ were compared and OR were calculated by using PCR-SSP to detected 31 alleles of HLA-DR gene sites and 8 alleles of HLA-DR gene sites of 146 tuberculosis cases and 146 controls from Shenzhen in China. Results GF of DRB1^＊1601-1605/1607-1608,DRB1^＊040101-44 of pulmonary tuberculosis group were strikingly higher than those of control group（P〈0.05）, its GF and OR were respectively 14.39% vs 8.59%, 19.33% vs 10.86%, 1.85 vs 2.08; There was strikingly difference in HF of DR4-DRB4 and DR16-DRB5 between pulmonary tuberculosis and control group, those of pulmonary tuberculosis group was evidently higher than control group and their OR was respectively 2.35 and 2.97. Conclusion The strikingly difference in HF of DR4-DRB4 and DR16-DRB5 between two groups maybe result from difference in GF of DRB1^＊1601-1605/1607-1608,DRB1^＊040101-44,and they may be the susceptive gene which led to pulmonary tuberculosis in Shenzhen.
出处
《基层医学论坛》
2006年第5期387-390,共4页
The Medical Forum
基金
深圳市科委资助项目(200204213)
