摘要
目的探索内蒙古地区汉族小儿支气管哮喘（BA）及其间歇发作和重度发作与HLA-DQA1等位基因的相关性,以寻找相关基因,探寻其部分发病机制及防治前景。方法采用病例对照研究策略,引入聚合酶链反应-序列特异引物法技术,选择小儿BA 66例为病例组（间歇发作42例,重度发作24例）和96名健康小儿,作HLA-DQA1等位基因的型别比较分析。基因频率比较在单因素四格表χ2或Fisher检验的基础上作多因素Logistic回归分析。结果病例组小儿BA、间歇发作和重度发作的HLA-DQA1＊0401基因频率分别为9．1%、9．5%和8．3%．与对照组的1%比较,χ2分别为12．260、9．849、—,P值分别为0．000、0．002和0．016。回归后,Wald分别为6．836、5．469和6．237,P值分别为0．009、0．019和0．013,均P＜0．05。B分别为2．048、1．923和2．205．均B＞0,OR分别为7．754、6．844和9．072,均OR＞1,其95%可信区间分别为1．670～36．005、1．365～34．310和1．607～51．212,其内均不包含1。EF分别为0．746、0．683和0．593,均EF＞0;而病例组小儿BA及其间歇发作的HLA-DQA1＊0103基因频率分别为7．6%和4．8%,与对照组的17．7%比较,χ2分别为6．843和8．250．P值分别为0．009和0．004。回归后．Wald分别为5．841和6．898,P值分别为0．016和0．009,均P＜0．05。B分别为-0．967和-1．469．均B＜0,OR分别为0．380和0．230,均OR＜1,其95%可信区间分别为0．174～0．833和0．077～0．689,均内均不包含1。PF分别为0．271和0．260,均PF＞0。结论HLA-DQA1＊0401等位基因可能是内蒙古地区汉族小儿BA及其间歇发作和重度发作发病单体型中的一个遗传易感基因,而HLA-DQA1＊0103可能为小儿BA及其间歇发作的保护基因。
Objective To explore the correlation between HLA-DQA1 allele and brochila asthma（BA） and its intermittent out break and grave outbreak in children of Hans in Inner Mongolian, and to find correlated genes and discuss part of pathogenesis and explore the prospect of prevention and cure of BA. Methods The method of case control was adopted. 66 children BA（42 cases of intermittent outbreak and 24 cases of grave outbreak） were selected as case group and health children 96 as control group in Hans,who had resided in Inner Mongolia three generation without consanguinity, history of mixed marriages, other medical history, and family history of immunily,led into PCR-SSP technique. The comparing of gene frequencies were dealt with by Logistic regression after one-way χ2 or Fisher test. Results The gene frequencies of HLA-DQA1 ＊ 0401 allele in children BA（9.1% ） ,intermittent outbreak（9.5 % ） and grave outbreak（8.3 % ） compard to control groups 1% , χ2 were 12. 260,9. 849 and -, P were 0. 000,0. 002 and 0. 016 respectively. After regression Wald were 6. 836,5. 469 and 6. 237, P were 0. 009,0.019 and 0. 013, P 〈 0.05 for all. The difference had statistical significance. B were 2. 048,1. 923 and 2. 205, B 2〉 0 for all. OR Were 7. 754,6. 844 and 9. 072, OR 2〉 1 for all and they were led to pathogenesis, whose 95 % confident interval were 1. 670 -- 36. 005,1. 365 -- 34. 310 and 1. 607 -- 51. 212 and which did not include 1. EF were 0. 746,0. 683 and 0. 593 ,EF〉0 for all. While the gene frequency of HLA-DQA1 ＊ 0103 allele in children BA（7.6 % ） and intermittent outbreak（4.8% ） compard to control group（ 17.7 % ） ,χ2 were 6. 843 and 8. 250, P were 0. 009 and 0. 004. After regression Wald were 5. 841 and 6. 898, P were 0. 016 and 0. 009, P 〈 0.05 for all, difference had statistical significance. B were -0.967 and - 1.469.B〈0 for all,OR were 0.380 and 0.230,OR〈 1 for all and it was protective pathogenesis. Whose 95 % confident interval were 0. 174- 0. 833 and 0. 077 - 0. 689 and w
出处
《中国基层医药》
CAS
2007年第4期529-532,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
内蒙古自治区卫生厅资助项目（内卫发[2001]181号文件）
