摘要
目的研究人类自然抵抗相关巨噬细胞蛋白1(NRAMP1)基因D543N多态性与汉族脊柱结核易感性的关系。方法选取汉族脊柱结核患者54例,正常对照60例,用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)的方法对NRAMP1基因D543N进行基因分型,根据基因型对样本分组,经统计学处理,研究D543N多态性与脊柱结核易感性的关系。结果在脊柱结核病患者中G/G基因型有32例(59.3%),G/A基因型有21例(38.9%),A/A基因型有1例(1.8%);健康对照组的G/G基因型为48例(80.0%),G/A基因型为11例(18.3%),A/A基因型有1例(1.7%)。两组间比较,具有显著性差异(P<0.05)。杂合子(G/A)等位基因在脊柱结核患者组高于健康对照组。结论NRAMP1基因D543N多态性可能是脊柱结核的遗传易感因素。
Objective To determine whether D543N polymorphisms of the NRAMPI gene arc associated with tuberculosis in Hans. Methods D543N polymorphisms of NRAMP1 gene were typed by PCR - RFLP among 54 patients with spinal tuberculosis and 60 healthy individuals. The relationship between D543N polymorphisms and susceptibility to tuberculosis was studied, and cases were grouped according to genotypes. Results In the spinal tuberculosis patients, genotype G/G, G/A and A/A were observed in 32 （ 59.3% ） ,21 （ 38.9% ） and 1 （ 1.8% ） case, respectively. While the genotypes of the healthy controls were G/G in 48 （ 80.0% ）, G/A in 11 （ 18.3% ） and A/A in 1 （ 1.7% ） case. The frequency of the genotype G/A was found more often among patients than that in controls and significant association was observed between spinal tuberculosis and D543N polymorphisms （x^2 = 0. 049, P 〈 0. 05 ）. Conclusion Polymorphisms of D543N locus in NRAMP1 gene might affect their susceptibility to spinal tuberculosis in Han nationality living in China.
出处
《宁夏医学杂志》
CAS
2008年第4期303-304,共2页
Ningxia Medical Journal
