摘要
目的:研究苦瓜减肥降糖的炎性相关机理。方法:经12周高脂饮食(45%热量源自脂肪,Research Diets D12451),建立小鼠肥胖和糖尿病模型。在此基础上,将小鼠分为低脂组(10%热量源自脂肪,Research DietsD12450B)、低脂加苦瓜组(苦瓜干粉按质量比5%加入低脂饲料中)、高脂组(同造模时的高脂饲料)、高脂加苦瓜组(苦瓜干粉按质量比5%加入高脂饲料中),以考察6周苦瓜饮食干预的影响。测定小鼠体质量、血糖、血脂等各项代谢指标。利用Real-time PCR和Western blotting,考察炎性细胞浸润、炎性因子表达及相关信号活性的变化情况。结果:通过饮食补充苦瓜6周,高脂加苦瓜组与高脂组小鼠相比体质量下降了6g,血脂水平也显著降低,接近低脂饮食组;脂肪组织巨噬细胞和肥大细胞的特征基因F4/80和mMCP-6的表达减少到高脂对照组的1/4,炎性因子MCP-1、IL-6、TNF-α的表达也显著降低;各项糖脂代谢指标(胰岛素耐受和葡萄糖耐受)明显改善;糖摄入相关信号(AKT、PPARγ及Glut-4)显著增强。
Aim： The purpose of this study is to indicate the anti-obesity and anti-diabetes mechanism of bitter melon （BM） through an anti-inflammatory mechanism. Methods： Obese mouse models were induced by feeding high fat diet （45% calories from fat; Research Diets no. D12451） for 12 weeks. Then the mice were divided into four groups according to their diets （low fat diet, 10% calories from fat; Research Diets no. D12450B; low fat diet supplemented with 5% BM; high fat diet; high fat diet supplemented with 5% BM）： LFD, LFD ＋ 5% BM, HFD and HFD ＋ 5% BM, respectively. Six weeks later, body weight gain, blood glucose, blood fat and other metabolic indices were investigated. Using qPCR, ELISA and Western blotting, we also explored inflammatory cell infiltration, inflammatory cytokine expression and related signal pathways. Results： After dietary supplementation of BM, body weight and blood fat of obese mice were dramatically reduced almost to those in the LFD group. The expression of F4/80 and mMCP-6, characteristic genes of macrophage and mast cell, and inflammation cytokines （MCP-1, IL-6 and TNF-ct） in adipose tissue were decreased sharply. The metabolism index of glucose and fat also returned to normal level, and glucose intake related signals （AKT, PPARγ and Glut-4） were enhanced. This study not only explains how BM ameliorates diet-induced obesity and diabetes, but also supplies a preliminary guideline for the development of anti-obesity and anti-diabetes functional foods with BM.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第15期246-251,共6页
Food Science
基金
国家自然科学基金面上项目(31171315)
合肥工业大学青年教师创新项目(2012HGQC0019)
关键词
苦瓜
肥胖
胰岛素抵抗
炎性细胞
炎性因子
Momordica charantia
obesity
insulin resistance
inflammatory cells
inflammatory cytokine
