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家蚕斑纹突变基因(q)的SSR标记定位分析 被引量:1

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摘要 对家蚕幼虫斑纹突变基因(q)进行分子标记定位,有助于对性状的分子标记辅助选择和对该基因的定位克隆研究。利用家蚕雌性染色体在减数分裂过程中不发生交换的特点,采用幼虫斑纹为鹑斑(q)的家蚕品系g03和幼虫斑纹为素斑(p)的家蚕品系C108,组配正反交群体g03♀×(g03×C108)F1♂和(g03×C108)F1♀×g03♂,分别记作BC1M和BC1F。用已经构建的家蚕SSR分子标记连锁图谱中第7连锁群上的12对SSR标记引物在亲本间进行多态性筛选。BC1F群体中的普斑个体均表现出与(g03×C108)F1相同的杂合型带型,而所有个体的带型与亲本g03一致,为纯合型。结果筛选出S0703、S0707和S0710共3个与q基因连锁的SSR标记。利用BC1M群体构建了家蚕q基因及其连锁的SSR标记遗传连锁图,图距为18.0 cM,3个SSR标记及q基因的排列次序为S0703-q-S0707-S0710,q位于4.5 cM处,与q基因最近的标记为S0703,遗传距离为4.5 cM。
出处 《江苏农业科学》 北大核心 2013年第7期15-18,共4页 Jiangsu Agricultural Sciences
基金 国家现代农业产业技术体系建设专项(编号:CARS-22) 江苏省镇江市科技支撑计划(农业)项目(编号:NY2010026)
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