摘要
为挖掘茶树农艺性状相关的分子标记位点,选用38对SSR引物对43份福建茶树品种进行多态性扫描与群体结构分析。在群体遗传结构分析的基础上采用Tassel GLM(general linear model)和MLM(mixed linear model)方法进行标记与农艺性状的关联分析。共检测出238个等位变异,每个位点6.26个等位变异。供试群体的Shannon指数平均值为1.261。通过群体遗传结构分析将供试材料划分成3个亚群。以GLM分析,发现8个与种径、茶类、百粒重和花瓣数相关联的标记,对表型变异的解释率分别54.3%、13.3%~21.8%、57.2%和50.9%;以MLM分析,发现6个与种径、茶类和百粒重相关的标记,各标记对表型变异的解释率分别为54.2%、19.7%~24.2%、31.1%。
To identify the molecular marker loci related to specific tea agronomic traits,38 simple sequence repeat(SSR)markers were selected for the polymorphism detection and population structure analysis on 43 tea varieties found in Fujian.The population structure was analyzed based on the polymorphic SSR markers,while the association between the markers and agronomic traits was determined by fitting the data to TASSEL general linear model(GLM)and mixed linear model(MLM).A total of 238 alleles were detected in the 43 tea varieties with an average count of 6.26.The averaged Shannon's index was 1.261.The structure analysis showed 3 genetic subpopulations for the varieties tested.The association of markers and traits based on GLM showed 8 SSR markers relating to the seed stem,tea type,hundred-grain weight and flower petal count of a plant,with the phenotypic contributions of 54.3%,13.3%~21.8%,57.2%,and 50.9%,respectively.Whereas,MLM indicated that 6 SSR markers closely related to the seed stem,tea type,and hundred-grain weight,with the phenotypic contributions of54.2%,19.7%~24.2% and 31.1%,respectively.
出处
《茶叶学报》
2017年第3期115-120,共6页
Acta Tea Sinica
基金
福建省现代农业(茶叶)产业技术体系建设项目(2014~2017)
福建省属公益类科研院所基本科研专项(2015R1012-7
2017R1012-6)
福建省自然科学基金项目(2015J01098)
福建省农业科学院茶树种质资源创新团队项目(STIT 2017-3-12)
关键词
茶
SSR
群体遗传结构
关联分析
tea plant
SSR
population structure
association analysis
