摘要
目的:探讨桂皮酸(cinnamic acid,CA)联合顺铂(cisplatin)对人肝癌细胞株MHCC97的增殖抑制及凋亡诱导作用。方法:以人肝癌细胞株MHCC97为研究对象,分为正常对照组、CA组、cisplatin组和CA+cisplatin组。采用MTT比色法检测细胞活性,倒置显微镜下观察细胞凋亡形态学变化,膜联蛋白V/碘化丙啶双染流式细胞术定量检测细胞凋亡率,Western blotting法检测凋亡蛋白caspase-3的活化。结果:CA单用或与cisplatin联用对正常人肝细胞L-02未见明显抑制作用,但两药单用或联用可明显抑制肝癌细胞MHCC97的增殖并诱导其凋亡。CA单用或与cisplatin联合作用于MHCC97细胞,其早期凋亡率比cisplatin组高,而中晚期凋亡及坏死率却相反。联用组在低浓度时具有很好的促凋亡作用,其促凋亡活力强于CA或cisplatin单用组,且联用可起到显著减毒增效的作用。CA和cisplatin单用或联用均可促进caspase-3蛋白的活化,具有时间正效应,且CA组和联合组的cleaved caspase-3蛋白表达量高于cisplatin组。结论:CA和cisplatin联合应用或单用均能有效抑制MHCC97细胞增殖,诱导其凋亡,其机制可能与激活caspase-3表达有关。
AIM： To investigate the effects of cinnamic acid（CA） combined with cisplatin on the proliferation and apoptosis of human hepatocellular carcinoma cell line MHCC97.METHODS： Human hepatocellular carcinoma cell line MHCC97 was culured and divided into CA group,cisplatin group,CA ＋ cisplatin group and control group.MTT assay,inverted microscopy,annexin V-FITC / PI staining and flow cytometry were applied to identify the viability,morphology and apoptosis of the cells.The apoptosis-related signaling protein caspase-3 was detected by Western blotting.RESULTS： CA and cisplatin either alone or in combination significantly inhibited the proliferation and induced obvious apoptosis of MHCC97 cells,while CA alone or combined with cisplatin had no significant inhibitory effect on normal human liver L-02 cells.The rates of mid-and late apoptosis or necrosis were higher in cisplatin group than that in CA group or combination group,but the early apoptotic rate was just the opposite.Pro-apoptotic activity in combination group was much stronger than that in CA group or cisplatin group at lower concentration,and combination group promoted apoptosis and decreased the cytotoxic side effects of cisplatin.CA and cisplatin either alone or in combination also up-regulated the cleaved caspase-3 expression in a time-dependent manner,and the effects in CA group and combination group were higher than that in cisplatin group.CONCLUSION： CA and cisplatin either alone or in combination inhibit the growth of MHCC97 cells by inducing apoptosis,and the activation of caspase-3 may play important roles in these processes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第7期1219-1224,共6页
Chinese Journal of Pathophysiology
关键词
桂皮酸
顺铂
细胞增殖
细胞凋亡
肝肿瘤
Cinnamic acid
Cisplatin
Cell proliferation
Apoptosis
Liver neoplasms
