摘要
目的:通过对不同入药方式下三七中有效成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和类金属砷的含量进行测定,研究不同入药方式对三七安全有效利用的影响。方法:三七生粉试样按照直接入药、水提取和乙醇提取3种方式进行前处理,经湿法消解后,采用氢化物发生原子荧光法(AFS)测定砷含量;采用高效液相色谱法(HPLC)测定有效成分含量,色谱柱为Agilent TC-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.05%磷酸(B)进行梯度洗脱,检测波长为203 nm,流速1.0 mL.min-1,柱温30℃。结果:乙醇提取液的砷含量最少,人参皂苷Rg1、人参皂苷Rb1含量最多。结论:乙醇回流的提取方法与水煎煮的提取方法相比,其砷含量较低(平均值分别为0.023 5,0.110 8 mg.kg-1)且保留更多药效成分含量(总皂苷含量平均值分别为9.098%,7.738%),可能可代替水煎煮的提取方法。
Objective： Using the HPLC and atomic fluorescence spectrometry,to analyze notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and the total arsenic content in Panax notoginseng and its two extracting solutions.Method： The chromatographic separation was achieved on an Agilent TC-C18（4.6 mm × 250 mm,5 μm） column with acetonitrile（A）-0.05% phosphoric acid（B） as mobile phase for gradient elution at the flow rate of 1.0 mL.min-1.The column temperature was 30℃ and the detection wavelength was set at 203 nm.Treat Panax notoginseng with different extracting manners（unprocessed,traditional decocting and refluxing by ethanol）.Run wet digestion process before measuring the fluorescence intensity,and then get the content of total arsenic.Result： The content of total arsenic differs in different extracting manner,unprocessed traditional decocting refluxing by ethanol.Conclusion： Panax notoginseng refluxing by ethanol can extract more pharmaceutical components with low total arsenic.The traditional decocting could be replaced by the ethanol way.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期128-131,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央高校基本科研业务费专项资金
