摘要
采用不同剂量单核细胞增多性李斯特菌(Listeria monocytogenes,LM)LM90SB2腹腔注射感染小鼠219只,观察小鼠临床症状,纪录死亡情况,在感染后不同时间采集小鼠脑组织,进行脑组织中细菌回收、鉴定及病理组织学观察。结果显示,不同感染剂量感染小鼠,其临床症状和脑组织病理变化基本一致。但是随着感染剂量的增加,其临床症状出现时间逐渐缩短,死亡率和脑组织中细菌分离的时间明显不同。1/2LD50剂量组小鼠未出现死亡,2/3LD50组和LD50组死亡率分别为12.5%和63.3%;另外1/2LD50组感染后24h开始从脑组织中离到LM,96h所有感染小鼠脑组织均能分离到LM,2/3LD50组感染后12h开始能分离到LM,72h所有脑组织均能分离到LM。从小鼠临床症状及死亡率、脑组织细菌的回收情况和脑组织病理组织学变化综合考虑,以2/3LD50LM LM90SB2腹腔感染小鼠为动物感染模型。
In order to establish Listeria monocytogenes （LM90SB2） mice encephalitis model, using different dose intraperitoneal injection to 219 mice, observe clinical symptoms in mice, record of death. At different time after infection, collecting mice brain, isolating brain tissue bacteria and the pathological and histological observation. The research results are as follows.with different infec- tious dose,the clinical symptoms and pathological changes in brain tissue of mice is similar. But with the increase of infectious dose, the duration of the clinical symptoms gradually shortened, mortality and time of separation bacteria from brain tissue were markedly different. 1/2 LDs0 dose group of mice did not appear death,two-thirds LD50 group and the LD50 group mortality rates were 12.5% and 63.3% respectively;1/2 LD50 infected mice,could isolate LM at 24 h,and all brain tis- sue could isolate LM at 96 h;2/3 LD50 infected mice,could isolate LM at 12 h,and,all brain tissue could isolate LM after 72 h. Based on above results, the encephalitis model could be duplicated with two-thirds LD50 LM90SB2 intraperitoneal injection in mice.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第7期1032-1036,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31160506)
