摘要
为探讨Ca2+/CaM信使系统对渗透胁迫下小麦幼苗生理特性的调控效应,以豫麦49-198为材料,研究了Ca2+通道阻断剂异博啶(VP)和CaM拮抗剂盐酸氯丙嗪(CPZ)对PEG胁迫下小麦幼苗相对含水量、丙二醛(MDA)、超氧阴离子(O2.-)、双氧水(H2O2)、可溶性糖、可溶性蛋白质、脯氨酸和谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响。结果表明,随着渗透胁迫时间延长,VP和CPZ处理提高了小麦幼苗MDA、O2.-和H2O2含量,降低了相对含水量、可溶性糖、脯氨酸、可溶性蛋白质和GSH含量,抑制了SOD、POD和CAT活性,而且VP和CPZ复合处理对小麦幼苗的伤害程度更大。试验表明,Ca2+/CaM信使系统可能通过提高渗透调节物质和抗氧化剂含量、增强抗氧化酶活性、降低膜质过氧化水平调节小麦幼苗对渗透胁迫的适应性。
In order to investigate regulation effect of calcium/calmodulin on physiological traits in wheat seedling under osmotic stress,the experiment was carried out in laboratory to study the effects of calcium channel blockers VP and calmodulin antagonists CPZ on relative water content,malondialdehyde content（MDA）,superoxide anion free radical content（O·-2）,hydrogen peroxide content（H2O2）,soluble sugar content,soluble protein content,proline content,glutathione content（GSH） and activities of superoxide dismutase（SOD）,catalase（CAT） and peroxidase（POD） in wheat seedling under PEG stress.The results showed that VP and CPZ treatments were increased the MDA,O·-2 and H2O2 contents,decreased the relative water content,soluble sugar content,proline content,soluble protein content and glutathione content（GSH） and decreased the activities of SOD,CAT and POD in wheat seedling.At the same time,the injury to seedlings was more serious under compound treatment of VP and CPZ.These results indicated that calcium/calmodulin messenger system might regulate the drought resistance of wheat seedlings by affecting the content of osmotic adjustment and antioxidant substance,the activity of some antioxidant enzymes and cell membrane permeability.
出处
《水土保持学报》
CSCD
北大核心
2013年第3期204-208,共5页
Journal of Soil and Water Conservation
基金
河南省重点科技攻关项目(102102110030)
河南省教育厅科技攻关项目(12B210002)
关键词
小麦幼苗
渗透胁迫
CA2+
CaM信使系统
生理特性
wheat seedling
osmotic stress
calcium/calmodulin messenger system
physiological traits
