摘要
研究Ca2+.CaM是否参与小麦抗叶锈病反应的过程,为更深入了解小麦抗叶锈病的反应机制奠定基础。采用离体培养的方法测定小麦种子经CaM拮抗剂TFP(三氟拉嗪)、CPZ(氯丙嗪)和W-7(N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide)以及CaCl2预处理后小麦叶片接种叶锈菌后在不同时间点的酶活性。另外用实时定量PCR的方法检测小麦接种亲和小种和非亲和小种后CaM不同亚型基因的表达情况。试验结果表明:小麦叶片接种非亲和性叶锈菌后,TFP、CPZ和W-7浸种处理抑制了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升;小麦叶片接种亲和性叶锈菌后,CaCl2预处理加剧了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升。这说明Ca2+.CaM信使系统可能在小麦抗叶锈病过程中起着重要作用,并且不同的CaM亚型可能调控不同的抗病途径。
Investigate if CaM is involved in the defense responses of wheat to Puccinia triticina,and provide more evidences to the next research.After being blocked Ca2＋·CaM messenger transduction by pretreating with CaM antagonists TFP（trifluoperazine）,CPZ（chlorpromazine） and W-7 [（N-（6-aminohexyl）-5-chloro-1naphathalene sulfonamide）]and activated with pretreatment of CaCl2,the activities of peroxidase（POD）,phenylalanine ammonialyade（PAL）,polyphenoloxidase（PPO） and catalase（CAT） were measured in wheat leaves inoculated with Puccinia triticica.Addition,the expression of the CaM gene was tested by Real-Time Fluorescent Quantitative PCR after inoculating virulent race and avirulent race respectively.The results showed that the pretreatment with CaM repel reagents TFP,CPZ and W-7 inhibited the increase in the activities of POD,PAL,PPO and CAT after the inoculation with 05-5-127③ in detached wheat leaves.The pretreatment with CaCl2 enhanced the increase in the activities of POD,PAL,PPO and CAT after the inoculation with 05-22-64① in detached wheat leaves.And there is differential expression of CaM isforms genes under different interactions in wheat after inoculation.The results indicated that Ca2＋·CaM messenger might play an important role in the interaction of wheat and pathogen,and different CaM isforms might play different role.
出处
《华北农学报》
CSCD
北大核心
2008年第B10期234-239,共6页
Acta Agriculturae Boreali-Sinica
基金
国家“973”前期项目(2005CCA01600)
