摘要
目的:研究芦荟大黄素对脂多糖(lipopolysaccharide,LPS)介导的小鼠腹腔巨噬细胞的增殖、吞噬及高迁移率族蛋白B1(high mobility group box-1 protein,HMGB1)转位的影响。方法:常规分离培养小鼠腹腔巨噬细胞,以不同浓度芦荟大黄素(终浓度为10 mol/L,20 mol/L,40 mol/L,80 mol/L)或联合1 g/ml的LPS处理细胞,以MTT法检测细胞增殖;以中性红吞噬试验和免疫荧光染色法分别检测细胞的吞噬功能和细胞内HMGB1的转位情况。结果:(10～80)mol/L浓度范围内的芦荟大黄素无细胞毒性,但可呈剂量依赖性抑制LPS介导的细胞增殖,且在24h后达到高峰;各浓度组芦荟大黄素处理细胞24h后,可明显降低小鼠腹腔巨噬细胞吞噬中性红的能力,与LPS组相比有显著差异性;免疫荧光检测显示伴随芦荟大黄素浓度的增高,细胞核内的HMGB1的绿色荧光逐渐增强,而胞质内的HMGB1荧光不断减弱;各浓度组芦荟大黄素呈明显剂量依赖性的降低细胞质HMGB1蛋白灰度值,与LPS组相比有显著差异性。结论:芦荟大黄素可以抑制LPS介导的小鼠腹腔巨噬细胞的增殖、吞噬及HMGB1转位,并呈剂量依赖性。
To investigate the effects of aloe-emodin（AE） on the proliferation, phagocytusis and High Mobility Group Box-I （HMGBI） translocation from the nucleus to the cytoplasm in mouse peritoneal macrophages induced by lipopelysaccharide （LPS）. Methods： Different concentrations of aloe-emodin （10 mol/L,20 mol/L, 40 mol/L, 80 mol/L） were added to the normal or LPS-activated mouse peritoneal mac- rophages. Metabolic activity of mouse peritoneal macrophages was measured by MTY assay at 12h,24h and 48h after aloc-emodin treatment. Phagncytosis and the translocation of HMGB1 in mouse peritoneal macrophages were analyzed by neutral red phagncytosis and immunofluores- cence test respectively after 24h treatment by aloe-emodin. Results： The MTr assay showed aloe-emodin had no significant effect on the ac- tivity of nomal mouse macrophages at the dose of （ 10 - 80） mol/L. However, aloe-emodin at the dose of （20 - 80） mol/L markedly inhibi- ted LPS-activated mouse macrophages proliferation in a dose dependent manner （P 〈0.05 or P 〈 0. O1 ）and this inhibitory effect reached the peak at 24h after aloe-emodin treatment. Meanwhile, aloe-emodin at the dose of （ 10 -80） mol/L exhibited the inhibitory effect on LPS-acti- vated mouse macrophages phagocytosis （ P 〈 0.01 ）. test showed that with aloe emodin concentration increased, the green fluorescence of HMGB1 was gradually strengthened in cell nucleus , while in cytoplasm, the green fluorescence of HMGB1 was gradual- ly weakened 24h after stimulated by LPS. Furthermore, aloe emodin at the dose of （20- 80） mol/L in a dose dependent manner reduced HMGB1 gray value in cytoplasm compared to LPS group（ P 〈 0.01 ） . Conclusion： Aloe emodin in a dose dependent manner inhibited the proliferation, phagocytosis and translocation of HMGB1 in mouse peritoneal macrophages induced by LPS.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第2期35-38,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金(No.30873415)
教育部博士学科点专项科研基金(No.20124425110010)资助项目
