摘要
目的:探索大鼠经阴道-宫颈途径无创式置入宫内节育器(IUD)的成模效果。方法:选择已生育SD雌性大鼠50只,随机分为5组。无创组、开腹组分别采用无创式(经阴道-宫颈)和开腹法(经腹腔-子宫)置入单侧宫腔含铜IUD;无创假手术组和开腹假手术组分别按上述方法置入IUD后立即取出;另设空白对照组。造模后第16天,观察IUD放置成功率、脱落率、子宫形态学和子宫内膜病理学改变、宫腔冲洗液中血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)浓度。结果:在IUD放置成功率方面,无创组、开腹组、假手术组均为100%;在IUD脱落率方面,无创组、开腹组分别为0、12.5%,差异无统计学意义(P>0.05),无创组有2例IUD前端铜丝逸进腹腔;无创法引起的子宫充血水肿和局部腹腔粘连明显轻于开腹法,差异均有统计学意义(P<0.05);较之空白对照组,无创法和开腹法TXB2浓度均明显降低、6-keto-PGF1α浓度明显升高,且开腹法较之无创法变化程度更为明显,差异均有统计学意义(P<0.01)。子宫内膜病理学观察,无创组和开腹组子宫内膜上皮明显缺损,炎细胞浸润,组织充血水肿伴增生,血管腔扩大,散在血栓,以开腹组的IUD置入端尤为明显。结论:模拟临床IUD避孕措施的动物造模有可行性,无创式造模大鼠能为IUD节育副反应研究提供有效的动物模型,且提示无创法较之开腹法能明显减轻IUD所致子宫内膜损伤。
Objective ： To explore the contraceptive model of non - invasive insertion of intrauterine device （IUD） in rats. Methods ： Fifty SD rat morns were assigned into non - invasive, invasive, non - invasive sham, invasive sham and blank con- trol groups randomly. An copper - IUD was placed into the unilateral uterus through the vagina - cervix way in the non - inva- sive group and through the intraperitoneal - uterus way in the invasive group. The IUD was removed immediately after the operation mentioned above was conducted in the non -invasive sham and the invasive sham groups. The sixth day after model- ing, the successful rate of insertion, the expulsion rate, uterine morphological changes and concentrations of TXB2 and 6 - ke- to - PGF1α in the uterine flushing were evaluated. Results ： The successful rates of insertion were all 100% in non - invasive, invasive and sham groups. The IUD expulsion rate of the noninvasive group was lower than that of the invasive group, while no significant difference was found （P 〉 0.05 ）. The peritoneal heterotopic was found in two rats. The extents of the uterine con- gestion and edema and the adhesion of abdominal organs in the non - invasive group were much less than those of the invasive group（P all 〈0.05）. The concentrations of TXB2 in the non -invasive group and the invasive group were significantly lower than that of the blank control group, while the concentrations of 6 - keto - PGF1α were much higher （P 〈 0.01 ）. Endometrial epithelial defects, inflammatory cell infiltrates and scattered thrombus were obvious in the non- invasive group and the inva- sive group, especially in the site of IUD insertion of the invasive group. Conclusion： It is feasible to simulate clinical IUD insertion for contraception in the rat, and the rat model could be used for the study on side effects of IUD use And less damage on the endometrium with the non -invasine approach is suggested.
出处
《中国计划生育学杂志》
2013年第7期466-471,共6页
Chinese Journal of Family Planning
基金
国家自然科学基金项目(81001536)
教育部博士点基金项目(20103731120003)
山东省自然科学基金项目(ZR2010HQ018)
关键词
宫内节育器
避孕
大鼠
无创式建模
动物模型
Intrauterine device
Contraception
Rat
Non - invasive modeling
Animal model
