摘要
在考察单因素(甲醇浓度、提取时间、提取温度和料液比)实验条件对木豆叶中球松素提取率影响的基础上,采用正交实验设计法优化得到球松素的最佳提取工艺条件,同时通过DPPH自由基清除实验评价了提取物的抗氧化活性。结果表明,木豆叶中球松素超声提取的最佳工艺条件为,甲醇提取浓度80%,提取时间60min,提取温度50℃,料液比1∶40(g/mL),球松素的提取率可达到1.832mg/g;甲醇提取物清除自由基的IC50值为0.281mg/mL。本研究建立的木豆叶中球松素的超声提取工艺具有提取效率高、操作简便等优点,适合木豆叶中球松素的提取,且甲醇提取物具有良好的抗氧化活性。
Based on single-factor experimental results, effects of four parameters including methanol concentration,extraction time,extraction temperature and ratio of solid to liquid on the extraction yield of pinostrobin from pigeonpea leaves were optimized by orthogonal test design,and the antioxidant capacity of methanol extract （ME） was evaluated by 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging ability. Optimal extraction conditions were obtained as follows.methanol concentration 80% ,extraction time 60min, extraction temperature 50;C and solid-liquid ratio l：40（g/mL）. Under optimum conditions,the extraction yield of pinostrobin reached 1.832mg/g,and ME of pigeonpea leaves exhibited good antioxidant activity with an ICso value of 0.281mg/mL. Ultrasonic-assisted extraction technology established in the study possessed the advantages of high efficiency and ease of operation,which was suitable for the extraction of pinostrobin from pigeonpea leaves.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第14期224-228,共5页
Science and Technology of Food Industry
基金
中央高校基本科研业务费专项资金资助(DL13CA02)
公益性行业科研专项经费项目(201204601)
哈尔滨市科技创新人才专项资金(2011RFQXS074)
关键词
木豆叶
球松素
正交设计
超声提取
抗氧化
pigeonpea leaves
pinostrobin
orthogonal design
ultrasonic-assisted extraction
antioxidant activitiy
