摘要
建立了HPLC(高效液相色谱 )法测定兔血清中盐酸沙拉沙星的含量。固定相 :色谱柱 ,HypersilC18柱 ,1 0 μm ,Φ4.6× 2 0 0mm。保护柱 ,HypersilC18柱 ,1 0 μm ,Φ4.6× 30mm。均由大连依利特科学仪器有限公司购进。流动相 :0 .0 1 %三氟乙酸———乙腈——— 3%四丁基溴化铵 (6 0∶2 5∶1 5v/v/v)流速 :1 .0ml/min。保留时间 :3.2～ 3.8min。荧光检测器检测波长 :激发波长Ex为 2 98nm ,发射波长Em为 46 0nm。在这个系统中 ,盐酸沙拉沙星的色谱峰没有受到杂质的干扰。在0 .1～ 1 0 μg/ml范围内 ,浓度与峰面积具有良好的线性关系 (r=0 .997) ,盐酸沙拉沙星的平均回收率为 90 .7% (n =6 ) ,精密度CV % 2 .0 2 % (n =4) ,最低检测限为 0 .0 1 μg/ml。
A HPLC method for the determination of sarafloxacin hydrochloride was established. The Hypersil C 18 column was adopted both as the analytical column (10μm,φ4.6 × 200 mm) and guard column (10μm,φ4.6 × 30 mm).The determination conditions were: mobile phase 0.01% trifluoacetic acid-acetonitrile-3% tetra-butabromoammonium (60∶25∶15,V/V/V); flow rate 1.0 ml/min.; retention time 3.2～ 3.8 min; excitation wavelength 298 nm; and emission lengthwave 460 nm. No interference occurred in the area of sarafloxacin hydrochloride on the chromatogram. In the investigated area (0.1～10 μg/ml), sarafloxacin hydrochloride concentration showed a significant linear correlation with peak area (r=0.997).This method gave a high recovery of sarafloxacin hydrochloride (90.7%, n=6), an average accuracy of 2.02% (c.v.%, n=4) and a detection limit of 0.01 μg/ml in rabbit serum.
出处
《西南农业大学学报（自然科学版）》
CSCD
2000年第3期219-221,共3页
Journal of Southwest Agricultural University
