摘要
Tb3+和沙拉沙星(SRFX)反应生成二元配合物,发射Tb3+的特征荧光。人血清白蛋白(HSA)能够增强Tb3+-SRFX配合物的荧光强度,据此,建立了荧光法测定HSA的新方法。在最佳测定条件下,当HSA的浓度在0.50～90.0 mg/L时,Tb3+-SRFX-HSA体系荧光强度的增强和HSA的浓度有良好的线性关系,方法的检出限为0.13 mg/L。用该方法测定了人血清中HSA的含量,回收率在99.2%～99.6%之间。同时对荧光强度增强的机理进行了探讨。
Saraflaxcin can combine with Tb^3＋ to form complexes, which emit strong characteristic fluorescence of Tb^3＋. The fluorescence intensities of Tb^3＋ -SRFX complexes could be enhanced by human serum albumin （HSA）. Based on this phenomenon, a simple and sensitive fluorescence method for the determination of HSA was developed. Under the optimum conditions, the fluorescence enhancement of Tb^3＋ -SRFX complexes was proportional to the concentration of HSA in the range of 0.50 -90.0 rag/L, and the detection limit was 0. 13 mg/L. The method was applied to the determination of the content of HSA from human serum samples. The recoveries of 99. 2% - 99.6% showed that the method was accurate and reliable. Additionally, the mechanism of fluorescence enhancement was discussed.
出处
《分析试验室》
CAS
CSCD
北大核心
2013年第2期22-25,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(20875011)资助
关键词
铽
沙拉沙星
荧光
人血清白蛋白
稀土
Terbium
Saraflaxcin
Fluorescence
Human serum albumin
Rare earth
