摘要
目的 观察戊四氮点燃癫痫持续状态大鼠海马部位细胞外信号调节激酶（extracellular-regulated kinase ERK1/2）的变化规律及尼莫地平对其的影响.方法 7～8周龄清洁级健康雄性Sprague-Dawley （SD）大鼠,用随机数字表法分为3组：正常对照组（normal control,NC）35只、持续癫痫发作组（SE） 40只和尼莫地平干预组（NIM） 40只,采用腹腔注射戊四氮的方法制作癫痫持续状态模型,尼莫地平组大鼠在腹腔注射戊四氮前15 min给予尼莫地平腹腔注射（2.5mg/kg）,应用Western Blot的方法检测海马部位ERK的变化规律.结果 尼莫地平干预后,大鼠癫痫发作程度减轻,发作持续时间缩短.SE组大鼠海马部位p-ERK1/2表达存在动态变化.尼莫地平组大鼠海马部位P-ERK表达较癫痫组明显升高（3.26±0.95,2.56 ±0.82,P＜0.05）.结论 L型钙离子拮抗剂尼莫地平能够减轻戊四氮点燃大鼠的癫痫发作程度,其抗癫痫作用可能与海马部位ERK信号通路有关.
Objective To detect the expression of extracellular-regulated kinase （ERK） and phosphatedextracellular-regulated kinase （P-ERK） in the hippocampus after pentylenetetrazoloe-induced status epilepsy and the effects of nimodipine on it.Methods Male Sprague-Dawley adult rats （200-250 g） were randomly divided into normal control group（NC,n =35）,status epilepsy group （SE,n =40）,nimodipine group （NIM,n =40）.The rats were injected first with 40 mg/kg pentylenetetrazoloe（PTZ）,followed 10 minutes later by 20 mg/kg PTZ,and subsequently,10 mg/kg PTZ ip every 10 minutes until SE occurred,apoint charactered by a loss of postural control and tonic-clonic seizures.Rats in control group received the same number of saline injections.Rats in NIM group were injected NIM（2.5 mg/kg） intraperitoneally 15 min before the injection of PTZ.Rats in every group were killed at 30 minutes,1 hour,3 hours,12 hours,24 horus,72 hours and 7 days after status epilepsy respectively and the hippocampus were dissected.The expression of ERK and P-ERK in the hippocampus were detected by Western blot.Results Nimodipine attenuated the convulsion of PTZ-induced status epilepsy.There was dynamic expression of P-ERK in SE group.In NIM group,the expression of P-ERK was markedly increased than that of SE group at 30 min,1h,3h,12h,24h,72h,and 7d （3.26 ±0.95 vs 2.56 ±0.82 at 30 min,P〈0.05）.Conclusion Nimodipine attenuates the convulsion of PTZ-induced status epilepsy with increased expression of phosphated-ERK in the hippocampus of rats.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2013年第6期500-502,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
基金项目：河北省自然科学基金项目（2010000548）
