摘要
目的 探讨迷走神经、躯体神经（坐骨神经、三叉神经）与运动皮质电刺激对戊四氮点燃大鼠惊厥行为的影响是否存在差异.方法 分别剥离大鼠迷走神经、坐骨神经（三叉神经不予以剥离）和建立运动皮质电刺激模型,给予上述4种电刺激后腹腔注射戊四氮50 mg/kg,观察大鼠行为学的变化.结果 对照组在注射戊四氮后9只大鼠（9/10）出现Racine分级Ⅳ～Ⅴ级惊厥发作,迷走神经、坐骨神经、三叉神经刺激后大鼠惊厥发作均不同程度减轻（Ⅰ～Ⅲ级发作大鼠分别为5、6、5只）,但给运动皮质电刺激后大鼠无惊厥行为.当实验鼠的皮质、海马出现病理改变（实验鼠已成为癫（癎）模型）时,再给同剂量戊四氮点燃时,相同参数的运动皮质电刺激则不能抑制大鼠的惊厥行为,反而出现Ⅳ～Ⅴ级发作（10/10）.结论 在生理性状态下4种电刺激方法在行为学上均可不同程度抑制戊四氮诱发的惊厥发作,以皮质电刺激效果最好,然而当鼠脑内的癫（癎）灶形成且处于病理状态下时此种效果消失.
Objective To investigate whether there are different effects of electric stimulation of vagus nerve,peripheral nerve（sciatic nerve and trigeminal nerve）,and motor cortex on pentylenetetrazol （PTZ）induced convulsion in rats.Methods The vagus nerve and sciatic nerve were exposed in rats.The stimulation electrodes were placed on the vagus nerve,sciatic nerve,trigeminal nerve,and motor cortex,respectively.After electric stimulation,PTZ（50 mg/kg）was intraperitoneally injected into the rats.The pattern and latency of the convulsion seizure were observed and recorded.Results Racine＇s grade Ⅰ-Ⅴ grade convulsion seizure Was present in 9 rats（9/10）in the control group after the injection of PTZ.However,this intensity Was reduced to Ⅰ-Ⅲ grade differentially in all the rats by electric stimulation of the vagus nerve（5/10）or peripheral nerve（6/10 and 5/10）.Furthermore,in the group of rats stimulated at motor cortex,there Was completely no convulsion.On the other hand,when pathological changes appeared in cortex or hippocampus（i.e.epileptic model was set up by 7 weeks stimulation）,the same stimulation of motor cortex was not able to inhibit the convulsion seizure induced by injection of PTZ and all these rats showed Ⅳ-Ⅴ grade seizure（10/10）.Conclusions In physiological condition,all of the four types of stimulation differentially reduced intensity of convulsion seizure triggered by PIZ injection and motor cortex stimulation has the best effect.However.when rats were in pathological status and epileptic nidus appeared in their brains.stimulatiion of motor cortex has no effect on PTZ induced convulsion seizure.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2010年第7期469-472,共4页
Chinese Journal of Neurology
基金
山西省自然科学基金资助项目（2009011057-2）
关键词
发作
电刺激
点燃效应
神经病学
戊四唑
迷走神经
运动皮质
Seizures
Electric stimulation
Kindling,neurologic
Pentylenetetrazole
Vagus nerve
Motor cortex
