摘要
目的探讨咪达唑仑直肠给药短时间的吸收情况和抗惊厥效果,以及对海马神经元的保护作用。方法①咪达唑仑直肠给药后,采用高效液相色谱法测定人血清中的药物浓度,观察其吸收情况;②用直接电刺激大鼠皮层惊厥模型作为观察对象,动态观察咪达唑仑直肠给药30min内,11个时间段的药效和时效的变化,实验结束后与对照组比较其海马神经元的组织学变化。结果①咪达唑仑直肠给药吸收迅速,用药安全;②咪达唑仑、地西泮均能显著提高模型大鼠的惊厥阈值(P<0.05),但前者抗惊厥效应更强,能更快发挥作用;③咪达唑仑组的海马神经细胞损害较轻。结论咪达唑仑直肠给药后,几分钟内即可产生有效的抗惊厥作用,且对海马神经元产生保护作用,其作用强于地西泮。
Objective To study the acute absorption, anticonvulsive effect and hippocampal neuroprotection of per-rectal midazolam. Methods ① High performance liquid chromatography was used to examine the serem concentration and absorption time of per-rectal midazolam in human;② Using rat models of convulsion induced by direct cortical stimulation, the anficonvulsive effect and time-effect profile of per-rectal midazolam at 11 time-spots within the first 30 minutes following medication were observed. At the end of experiment, pathology of rat hippecampus was examined and compared with controls. Results ①Per-rectal midazolam was safe with quick absorption. ②While both of midazolam and diazepam elevated the convulsion threshold in rats （ P〈0.05 ）, the effect of former was more quickly and effectively. ③ The neuronal injury appeared milder in the group treated with per-rectal midazolam. Conclusion Per-rectal midazolam showed anficonvulsive effects within minutes after administration and provided stronger hippocampal neuroprotection as compared with diazepam.
出处
《中国药物与临床》
CAS
2006年第12期909-911,T0002,共4页
Chinese Remedies & Clinics
关键词
投药
直肠
发作
海马
咪达唑仑
Administration,rectal
Seirures
Hippocampus
Midazolam
