摘要
应用基因芯片检测技术研究大强度耐力训练对大鼠脑组织糖代谢和脂肪酸代谢相关基因表达的影响.将SD大鼠随机分为安静组与大强度耐力训练组,两组自由摄食和饮水,安静组安静饲养,大强度耐力训练组训练7周后处死,迅速取出脑组织提取mRNA,作逆转录为cDNA以备检测.利用基因芯片技术初步筛选出与运动能力相关的糖代谢和脂肪酸代谢有关基因分别为4和8条,均表达下调.与糖代谢相关基因表达产物分别是乳酸脱氢酶同工酶3(LDH3)、UDP-葡萄糖苷酸(基)转移酶、葡萄糖6-磷酸酶和磷酸甘油变位酶;与脂代谢相关基因表达产物分别是羧酯脂肪酶、二乙基对硝基苯磷酸酯酶、硬脂酰CoA去饱和酶、羟基类固醇磺基转移酶、芳乙酰胺脱乙酰酶、载脂蛋白A(-I和-V)和溶血磷脂酸Δ-酰基转移酶.筛选出大强度耐力训练大鼠脑组织中与糖代谢和脂肪酸代谢过程相关基因,且均表达下调,说明运动训练已经对大鼠脑组织物质代谢和能量代谢产生抑制作用,这可能也是引起运动性中枢疲劳的机制之一.
The effect of genes metabolism in the brain of the expression connected with glycometabolism and fatty acid high-intensity endurance training rat was studied using cDNA microarray. Rats were divided into the quiet group and the strong intensity endurance training group at random. The both groups freely ingest and drink. The quiet group of rats was bred quietly. The group with high-intensity endurance training was put to death after training for 7 weeks. Brains were taken quickly to extract mRNA for reverse transcription cDNA for detection. There were the 4 and 8 strips of genes connected with glycometabolism and fatty acid metabolism expressed in rats＇ brain, respectively, and all were down-regulation. The genetic expression product related to glycometabolism were LDH3, UDP-glucuronosyltransferase, Glucose-6- phosphatase （G6pc）, phosphoglyceromutase, lipase, paraoxonase, stearyl-CoA desaturase, and fatty acid metabolism were carboxyl ester sulfotransferase, Apolipoprotein A （-I and -V）,arylacetamide deacetylase and lysophosphatidic acid acyltransferase-delta, separately. The genes connected with glycometabolism and fatty acid metabolism were screened in the brain of the high- intensity endurance training rat and all down-regulation. The results revealed that the high- intensity endurance training could restrain glycometabolism and fatty acid metabolism in the brain of rat, which was one of the biology mechanism of the center fatigue.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第3期104-108,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
陕西师范大学211工程重点研究资助项目(2010-93)
陕西师范大学体育学院青年教师科研基金资助项目(20131402)
关键词
大强度耐力训练
基因表达
糖代谢
脂肪酸代谢
脑组织
high-intensity endurance training
gene expression
glycometabolism
fatty acid metabolism
brain tissue
