摘要
以艾蒿嫩茎为材料,采用组织培养的方法,进行了试管苗培养、微型根茎试管苗培养、微型根茎苗床扦插等研究.结果证明:以具有2个生长点的微型根茎为材料,在1/2MS+IAA 0.2 mg/L+蔗糖20 g/L的培养基上试管苗微型根茎的繁殖的速度最快;以具有2个生长点的根茎段为材料、在以炉灰渣为扦插基质的温室苗床上对试管苗微型根茎进行扦插繁殖是微型根茎理想的繁殖方法.试管苗移植的成活率为98.75%.移植成活的微型根茎试管苗保持了野生艾蒿的所有植物学性状.
In order to preserve the wild resources and solve the problem of the slow speed of tube seedlings breeding, with the method of tissue culture,tender stems of Artemisia argyi were used as material to do the research on tube seedling cultivation, miniature rhizome test-tube seedling cultivation and miniature rootstock cutting seedbed. The results showed that in the media of 1/2MS＋IAA 0.2 mg/L＋sucrose 20 g/L, the propagation speed of miniature rootstock with two growing points was the fastest. The miniature rootstock with two growing points,using the furnace ash as the greenhouse seedling bed was an ideal propagation method. The transplanted survival rate of the test-tube seedling was 98.75%. The tube seedlings with the miniature rootstock which transplanted survival kept all botany traits of the wild A. argyi.
出处
《河南科学》
2013年第5期595-598,共4页
Henan Science
基金
辽宁省普通高等教育本科教学改革研究项目(201203041-4)
关键词
艾蒿
试管苗
微型根茎
Artemisia argyi
tube seedlings
miniature rhizome
