摘要
以透骨草的嫩茎为材料,进行了愈伤组织诱导、愈伤组织分化、不定芽生根、试管苗的移栽、试管苗扦插、试管苗移植的研究,成功的建立起透骨草的嫩茎无性系。结果证明：1/2MS＋BA0.5mg/L＋2,4-D1.2 mg/L＋NAA0.2-0.8 mg/L为嫩茎愈伤组织的的理想培养基;1/2MS＋AgNO31.2 mg/L＋BA0.6 mg/L＋NAA0.1 mg/L为愈伤组织和不定芽分化的理想培养基;1/3MS＋IAA0.4 mg/L为不定芽生根培养和生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。移植试管苗后期出现生长旺盛、长势整齐的性状,翌年春季出现萌发早5d、根系相当于野生植株2倍的性状。
The study on callus induction, differentiation, rooting and transplantation of tube seeding was taken by using young stem of Phryma leptostachya L. var. asiatica Hara as material. The asexual line of young stem was established The results indicated that the ideal medium for induction and differentiation of callus were 1/2MS＋BA 0.5 mg/L ＋2,4-D1.2 mg/L ＋NAA 0.2-0.8 mg/L and 1/2MS＋ AgNO3 1.2 mg/L＋BA 0.6 mg/L ＋NAA 0.1 mg/L respectively. The effective medium for rooting and regeneration was 1/3MS＋IAA 0.4 mg/L. The cinder was the optimum matrix of seedling transplanting. The seedling keeps the exuberant growth.
出处
《中国林副特产》
2009年第2期14-16,共3页
Forest By-product and Speciality in China
关键词
透骨草
组织培养
无性系
快速繁殖
Phryma leptostachya L. var. asiatica Hara Tissue culture
Clone
Rapid propagation
