摘要
目的:优化羟基自由基处理葡萄表面的腐败霉菌和酵母菌的灭菌条件,提高杀菌效率。方法:采用响应面法优化时间、电流强度、涌浪和菌浓度对杀菌效果的影响。结论:通过单因素试验发现涌浪对于杀菌率几乎无影响,确定出最佳杀菌时间为20min,最佳浓度为每500g葡萄接种菌15mL,最佳电流强度为4V。对由Design-Export设计出的17种组合进行试验,将得到的结果依据回归分析确定最佳杀菌条件,对霉菌最佳杀菌条件为电流强度7.17V、时间21.89min、接菌量15.15mL,理论上最高杀菌率为0.95813;对酵母菌最佳杀菌条件为电流强度7.27V,时间22.48min、接菌量14.56mL,理论上最高杀菌率为0.96673。
Objective： Optimization of hydroxyl radical treatment on grape surface corrupt moulds and yeasts sterilization conditions, to improve the sterilization efficiency. Method： Response surface methodology, current intensity, swell, the bacteria concentration on the bactericidal effect. Conclusion： The single factor experiment found that the surge sterilization rate has almost no effect, determine the optimum sterilization time of 20 min, the optimal concentration for each 500 g grapes inoculated with bacteria 15 mL, the best strength of 4 V. 17 kinds of combinations of the design by the Design-Export test will be in accordance with regression analysis to determine the optimal sterilization conditions, to mold the best sterilization conditions for strength 7.17 V, time 21.89 min, inoculum 15.15 mL, theoretically the highest sterilization rate of 0.95813; yeast optimal sterilization conditions for strength 7.27 V, time 22.48 min, inoculum 14.56 mL, in theory, the highest sterilization rate of 0.96673.
出处
《食品科技》
CAS
北大核心
2013年第5期52-58,共7页
Food Science and Technology
关键词
羟基自由基
霉菌
酵母菌
灭菌
响应面分析法
hydroxyl radicals
mould
yeast
sterilization
response surface analysis
