摘要
目的初步探究慢性阻塞性肺疾病急性加重期(AECOPD)大鼠模型气道黏液高分泌与肺功能的相关性。方法将40只Wistar雄性大鼠随机分为正常组和AECOPD模型组,建立大鼠AECOPD模型,用酶联免疫吸附法(ELISA)测定大鼠血清中中性粒细胞弹性蛋白酶(NE),并测定肺功能指标。结果模型组NE比正常组升高,差异有统计学意义(P<0.01)。模型组吸气阻力(Ri)、分钟通气量(MVV)、第0.3秒用力呼气容积与用力肺活量比值(FEV0.3/FVC)、呼气峰流速(PEF)与正常组比较,差异均有统计学意义(P<0.01)。NE水平与Ri呈正相关;NE水平与MVV、FEV0.3/FVC呈负相关;NE水平与PEF无相关。结论 AECOPD大鼠气道黏液高分泌产生过量的黏液,加重气道阻塞,导致严重的气流受限,从而加速了肺功能持续下降的进程,所以AECOPD持续存在的气道黏液高分泌与肺功能进行性下降存在密切的相关性。
Objective To discuss preliminarily the correlation between the high secretion of airway mucus and lung function in the rat model of acute exacerbation of chronic obstructive pulmonary disease（ AE- COPD）. Methods Forty Wistar male rats were randomized into a normal group and an AECOPD model group. AECOPD rat model was set up. The enzyme linked immunosorbent assay （ELISA） was used to deter- mine the level of serum neutrophil elastase （ NE ） in the rats. The indexes of pulmonary function were deter- mined. Results NE in the model group was increased as compared with the normal group, indicating the sta- tistically significant difference（ P 〈 0.01 ）. In the model group, the inhalation resistance（ RI）, minute ventila- tion volume （MVV）and the ratio of forced expiratory volume in 0.3 seconds and forced vital capacity（FVC） （FEV0.3/FVC） and peak expiratory flow（PEF） were all different significantly as compared with those in the normal group（P 〈 O. 01 ）. NE level was positively correlative with RI, was negatively correlative with MVV and FEV0.3/FVC and was irrelative with PEF. Conclusion The high secretion of airway mucus in AECO- PD rats produces excessive mucus, accelerates airway obstruction and results in serious airflow restriction so that the constant declining of pulmonary function is enhanced. In summary, the persistent high secretion of airway mucus of AECOPD is closely correlative with the progressive deelining of pulmonary function.
出处
《世界中西医结合杂志》
2013年第4期397-399,共3页
World Journal of Integrated Traditional and Western Medicine
基金
北京自然科学基金资助项目(No.7112073)
北京中医药大学自主选题资助项目(No.JYB22-JS056)
