摘要
目的：建立微波消解石墨炉原子吸收光谱法（GFAAS）测定大鼠组织中的微量硒的检测方法。方法：采用微波消解法处理样品,用硝酸作为消解剂进行微波消解;采用氯化钯则作为基体改进剂来测定样品中的微量硒。结果：最佳灰化温度为900℃,原子化温度为2500℃;脑组织和肺组织的相对标准偏差（RSD）分别为2.83%和3.05%,样品加标回收率为97%~105%。结论：该方法用于检测大鼠组织中的硒含量,操作简便、快速、准确度好、结果满意,能够适用于生物体内多种组织器官中硒含量的测定与分析。
Objective： To develop a method for determination of trace selenium in rat tissues by microwave digestiongraphite furnace atomic absorption spectrometry （GFAAS）. Methods： Microwave Digestion Treatment of samples with nitric acid as the digestion agent for microwave digestion; palladium chloride is used as a matrix modifier for determination of trace selenium. Results： The best ashing temperature was 900 ℃, the atomization temperature was 2500℃; brain and lung tissue relative standard deviations （RSDs） were 2.83% and 3.05%, respectively; sample recoveries were in the range of 97% to 105%. Conclusion： The method for the detection of rat tissue selenium content is simple, fast, with good accuracy, and these results are satisfactory. And thus, the method can be applied to many organs and tissues of organisms in determination of the content of selenium and analysis.
出处
《现代生物医学进展》
CAS
2013年第9期1671-1674,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81000858)
