摘要
目的探讨人肠道柔嫩梭菌(Clostridium leptum,C.leptum)类群与慢性牙周炎和2型糖尿病的相关关系。方法收集慢性牙周炎患者(CP组)、2型糖尿病患者(DM组)、伴2型糖尿病的慢性牙周炎患者(DM+CP组)及健康对照者(HC组)各20例的粪便标本。根据C.leptum类群16S rRNA序列设计特异性引物,以常规PCR产物经克隆后的质粒DNA为标准品,经梯度稀释后制备标准曲线。待测粪便标本提取细菌基因组DNA,应用实时定量PCR方法对C.leptum类群进行定量分析。结果 4组标本每克湿便中C.leptum类群的16S rRNA基因拷贝数的对数值(log copies/g):HC组(7.09±0.17)、CP组(6.31±0.58)、DM组(5.79±0.52)、DM+CP组(5.41±0.29)。与HC组相比,其他三组C.leptum类群基因拷贝数均明显减少(P<0.05)。结论 C.leptum类群可能与慢性牙周炎和2型糖尿病的发生、发展相关。
Objective To investigate the relationship between Clostridium leptum （ C. leptum） subgroup in the human gut, the periodontal disease and type 2 diabetes mellitus. Methods Fresh fecal samples were obtained from twenty patients with chronic periodontitis （CP group）, twenty patients with type 2 diabetes mellitus （DM group）, twenty patients with chronic periodontitis complicated by type 2 diabetes [ （DM ＋ CP） group] and twenty healthy control subjects （HC group）. Genus-specific primer of C. leptum subgroup was designed for 16S rRNA-targeted PCR. The standard curve of real-time quantitative polymerase chain reaction （RTQ-PCR） was generated from a grade-dilution of plasmid DNA which was cloned from PCR products. Bacterial genome DNA extracted from fecal samples was quantified by RTQ-PCR to analyze C. leptum subgroup amounts. Results Logarithm for 16S rRNA gene copies of C. leptum subgroup per gram feces in HC group, CP group, DM group and （DM ＋ CP） group were （7.09 ±0. 17）, （6.31 ±0.58 ）, （5.79 ± 0.52） and （5.41 ± 0.29 ）, respectively. Compared with HC group, the logarithm of the other three groups were dramatically decreased （ P 〈 0.05 ）. Conclusion C. leptum subgroup may be associated with the development and progression of chronic periodontitis and type 2 diabetes mellitus.
出处
《山东大学学报（医学版）》
CAS
北大核心
2013年第4期100-104,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(30171010,30371323)
山东省科技发展计划(2010GSF10267)
关键词
慢性牙周炎
2型糖尿病
柔嫩梭菌类群
实时定量聚合酶链式反应
Chronic periodontitis
Type 2 diabetes mellitus
Clostridium leptum subgroup
Real-time quantitative poly- merase chain reaction
