摘要
目的探讨在人胃腺癌组织中是否存在血管生成拟态(Vasculogenic mimicry,VM)及VM与HIF-1α表达的关系,以及HIF-1α表达的临床病理意义。方法收集临床病例资料完整的胃腺癌121例,利用CD34和PAS双重染色观察是否存在VM,然后对VM阳性组和阴性组进行HIF-1α染色,分析VM与HIF-1α表达的关系及HIF-1α表达与患者临床病理指标的关系。结果 121例胃腺癌组织中有44例(36.36%)存在VM。VM阳性组和VM阴性组HIF-1α蛋白表达阳性率分别为77.27%(34/44)和54.55%(42/77),两组间的差异有统计学意义(P<0.05);有、无淋巴结转移的胃腺癌组织HIF-1α表达阳性率分别为76.74%(66/86)和28.57%(10/35),两组间的差异有显著性(P<0.01);中、低分化胃腺癌组织中HIF-1α表达阳性率分别为50.85%(30/59)、74.19%(46/62),两组间的差异有显著性(P<0.01)。结论胃腺癌组织中存在VM,HIF-1α的表达可能促进VM的形成。VM与HIF-1α的表达可能是胃腺癌浸润、转移的重要生物学标志。
Objective To study the existense of vasculogenie mimicry （VM） in gastric adenocarcinoma （GAC）, the relationship between VM and HIF-la, and the clinical significance of HIF-1α. Methods We used CD34 and PAS （periodic acid-Schiff） dual staining to validate the existence of VM in GAC by collecting 121 samples of gastric adenocarcinoma and their clinical data. HIF-1α staining was done in VM positive and negative control groups. We analyzed the relationships between VM and HIF-la, and between HIF-1α and clinicopathologic data. Results In 121 gastric adenocarcinoma cases, 44 showed VM. The positive ratio in the VM positive control group was 77.27% （34/44）, while that of the VM negative control group was 54.55% （42/77） ; With a statistically significant difference （P〈0.05）. The HIF-1α positive ratio was 76.74% （66/86） in patients with lymph node metastasis, and 28.57% （10/35）in those without, with a significant difference （P〈0.01）. The HIF-1α positive ratio was 50. 85% （30/59） in patients with moderately differentiated gastric adenocarcinoma, and 74.19 % （46/62） in those of poor differentiation, also with a significant difference （P〈0.01）. Conclusion VM exists in GAC. HIF-1α expression might pro- mote the formation of VM. VM and HIF-1α expression might be important biological markers for invasion and metastasis of gastric carcinoma.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2013年第2期135-139,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽医学高等专科学校校级自然课题资助(KJ2011X06)
关键词
胃腺癌
血管生成拟态
缺氧诱导因子-1Α
免疫组化染色
Gastric adeno carcinoma
Vasculogenic mimicry （VM）
Hypoxia inducible factor 1α
Immunohistochemical staining
