摘要
目的探讨肝细胞肝癌(hepatocellular carcinoma,HCC)中存在血管生成拟态(vasculogenic mimicry,VM)的肿瘤组织E-钙粘蛋白(E-cadherin,E-cad)与无VM生成的肿瘤组织E-cad的表达情况,初步探讨恶性肿瘤VM的生成特点,存在VM结构的肿瘤易于复发和转移及与VM可能相关的原因。方法收集手术切除的肝细胞肝癌标本40例,制作石蜡切片,进行Hepatocyte、CD31免疫组化染色,筛选出存在VM的肿瘤病例,并对40例病例标本进行E-cad免疫组化染色及进行吸光度分析。对存在VM结构的肿瘤标本进行连续切片,E-cad免疫组化染色,对管道状VM及血管不同层面行吸光度分析,观察VM表达E-cad的连续性特征。结果17.50%(7/40)的HCC存在VM。VM阳性组较VM阴性组HCC的E-cad表达明显下降,其差异存在统计学意义(P<0.01)。VM结构较内皮性血管周围HCC的E-cad表达亦明显下降,其差异亦存在统计学意义(P<0.05)。结论HCCE-cad表达的下降,使恶性肿瘤细胞更易经拟态血管发生血道转移,同时使肿瘤细胞间附着结构松散,细胞间更易发生解离及自身细胞变性,提示内皮性肿瘤血管与VM分属不同的形成机制。
Objective To investigate the relationship between vasculogenic mimicry （VM） and the expression of E-cadherin （E cad） in hepatocellular carcinoma （HCC） cells, the characteristics of VM in malignant tumors and the role of VM played in metastasis and recurrence of tumors. Methods Forty samples of HCC were collected surgically and cut into paraffin-embedded sections. By using Hepatocyte and CD31 immunohistochemistry staining, the positive samples for VM were screened out. By using immunohistochemistry the expression of E-cad was detected in 40 cases of specimens. Serial sections of VM-positive samples were collected and subjected to E-cad immunohistochemistry. The successional expression of E-cad in VM and vessels at different layers was assayed. Results VM was found in 17.50% （7/40） of HCC patients. The expression of E-cad in HCC in VM positive group was significantly lower than in VM negative group （P〈0.01）. The expression of E-cad in HCC around VM was significantly lower than that around blood vessels （P〈0.05）. Conclusion The down-regulation of the E-cad expression in HCC can facilitate the bematogeneous metastasis of malignant tumors through VM, and simultaneously lead to the looser coherence between tumor cells, cell dissociation and degeneration, indicating different mechanisms of the formation of endothelial tumor vessels and VM.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第6期769-772,776,842,共6页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
肝细胞癌
血管生成拟态
E-钙粘蛋白
carcinoma, hepatocellular
vasculogenic mimicry
E-cadherin
