摘要
目的测定人参中的果胶含量。方法先除去人参中的可溶性糖,然后加入果胶酶将果胶分解,再采用-3,5-二硝基水杨酸比色法对果胶含量进行测定。结果测定人参中果胶含量的最佳条件为:供试品细粉500 mg,经80%乙醇水浴提取后,过滤,残渣加入含有果胶酶1 200 U的枸橼酸-枸橼酸三钠缓冲液50.0 mL(pH 3.5),50℃水浴中恒温加热6 h酶解。用DNS比色法测定酶解液中的果胶含量(以半乳糖醛酸计)。结论本法操作方便、准确,可用于人参果胶含量的检测。
OBJECTIVE To determine the pectin content in Panax ginseng.METHODS Soluble sugar was removed firstly,then pectinase was added for enzymolysis,and 3,5-dinitrosalicylic acid colorimetry was employed for determination of pectin.RESULTS The optimal conditions of determination were as follows: 500 mg of Panax ginseng powder was extracted with 80% alcohol in water bath at 80 ℃,then filtrated,and the residue was enzymolyzed with pectinase of 1 200 u at 50 ℃ for 6 h in 50 mL of 0.1 mol·L-1 citric acid-sodium citrate buffer(pH 3.5).The galacturonic acid from degraded pectin in Panax ginseng was determined with 3,5-dinitrosalieyfic acid colorimetry.CONCLUSION This method has been proved to be convenient and accurate,which can be applied to the determination of pectin content in Panax ginseng.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第8期589-592,共4页
Chinese Pharmaceutical Journal
基金
辽宁省科技厅科技创新团队项目(2008T117)
辽宁省高等学校优秀人才支持计划项目(2008RC34)
国家自然基金资助项目(81173499)
关键词
人参
果胶
酶解
DNS比色法
Panax ginseng
pectin
enzymatic hydrolysis
3,5-dinitrosalicylic acid colorimetry
