摘要
通过对246株芽孢杆菌进行筛选,以期获得对耐甲氧西林金黄色葡萄球菌(MRSA)具抑制作用的菌株,抑菌结果表明,对耐甲氧西林金黄色葡萄球菌有抑制作用的菌株有5株,其中菌株1012-4的抑菌活性最强;为确定菌株种属,对优选菌株进行形态特征观察、生理生化特征鉴定以及API50CHB鉴定,初步确定1012-4为枯草芽孢杆菌;为进一步确证菌株类型,实验采用了16S rDNA基因测序,序列结果表明,菌株1012-4为芽孢杆菌属枯草芽孢杆菌种;经反复确证,确定菌株1012-4为优势菌株,抗菌活性显著,为新型抗MRSA药物的研发提供了菌株来源。
In this research, strains with antibacterial activity on Methicillin-resistant Staphylococcus aureus.246 tested strains was screened and identified, Antibacterial test results showed that five strains had antibacterial activity, among which strain 1012-4 showed the strongest activity. Morphological, physiological and biochemical characterization, as well as API50 CI-IB method, were adopted to determine the type of strain 1012-4. Preliminary results suggested that the stain 1012-4 was of Bacillus subtilis species. Through 16S rDNA gene sequence alignment, the strain 1012-4 was further identified as the genus Bacillus and Bacillus subtilis species. Verification test confirmed that, the strong antibacterial activity of strain 1012-4. This research provided new strain source for the research and development of new anti-MRSA drugs.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第4期788-791,共4页
Modern Food Science and Technology
基金
科技部重大新药创制专项(2010ZX09401-306-2-23)
重庆市科委项目(2009GJF10023)
关键词
耐甲氧西林金黄色葡萄球菌
芽孢杆菌
筛选
鉴定
methiciUin-resistant Staphylococcus aureus
bacillus
screening
identification
