摘要
Batch fermentation of MTG by S.mobaraense WSH Z2 at various temperatures ranging between 25℃～35℃ were studied.At 30℃,MTG activity and cell dry weight were higher than others,reached 2.94 u/mL and 25.1 g/L respectively.At 32℃ or higher,lagged time was shorter,specific cell growth rate(μ cell )and specific MTG production rate(μ MTG )were higher at earlier stage of culture,while at mid and last stage,μ cell and μ MTG were higher at 28℃.So the following strategy of temperature control was obtained:0～18 h,culture was performed at 32℃,and after 18 h,the temperature was shifted from 32℃ to 28℃.The results of this strategy showed that maximal MTG activity and productivity reached 3.37 u/mL and 80.2 u/L/h respectively,average μ cell and μ MTG obtained 3.02 u/h/g DCW and 0.028 g/h/gDCW,respectively,higher than all of that at single temperature.It could be seen that MTG production could be improved obviously by this temperature control.
Batch fermentation of MTG by S.mobaraense WSH Z2 at various temperatures ranging between 25℃～35℃ were studied.At 30℃,MTG activity and cell dry weight were higher than others,reached 2.94 u/mL and 25.1 g/L respectively.At 32℃ or higher,lagged time was shorter,specific cell growth rate(μ cell )and specific MTG production rate(μ MTG )were higher at earlier stage of culture,while at mid and last stage,μ cell and μ MTG were higher at 28℃.So the following strategy of temperature control was obtained:0～18 h,culture was performed at 32℃,and after 18 h,the temperature was shifted from 32℃ to 28℃.The results of this strategy showed that maximal MTG activity and productivity reached 3.37 u/mL and 80.2 u/L/h respectively,average μ cell and μ MTG obtained 3.02 u/h/g DCW and 0.028 g/h/gDCW,respectively,higher than all of that at single temperature.It could be seen that MTG production could be improved obviously by this temperature control.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第6期759-762,共4页
Chinese Journal of Biotechnology
关键词
谷氨酰胺转氨酶
分批发酵
温度控制策略
Streptoverticillium mobaraense, transglutaminase,batch fermentation,strategy of temperature control
