摘要
为了提高γ-氨基丁酸(GABA)的发酵水平,在3.7L发酵罐中考察了不同操作条件(通气和pH控制)对短乳杆菌CGMCCNO.1306分批发酵生产GABA的影响。结果表明,不同操作条件对GABA的发酵产量有显著影响。好氧发酵有利于菌体的生长,最大菌体干重达到2.78g·L-1,而厌氧发酵有利于产物GABA的生成,发酵72h时GABA的产量达到23.94g·L-1。在兼性厌氧条件下,研究了pH控制对GABA分批发酵的影响,实验发现pH控制在5.0时,GABA产量最高,发酵72h时GABA的产量达到40.73g·L-1.对GABA的补料发酵进行了初步研究,发酵108h时GABA产量达到76.36g·L-1,分别比摇瓶发酵、厌氧发酵以及控制pH5.0发酵提高128.6%、219%和87.5%。
In order to enhance the γ-aminobutyric acid （GABA） production, the effects of operation conditions （aeration and pH） on GABA batch fermentation by Lactobacillus brevis CGMCC NO.1306 in a 3.7 L stirred fermenter were investigated. The results show that the aeration and pH have significant effects on GABA production. The aerobic cultivation is advantageous to obtain high yield of dry cell weight （2.78 g·L^-1）, but the anaerobic cultivation is advantageous to GABA accumulation, and its GABA yield reaches 23.94 g·L^-1 at 72 h. In order to assess the effects of pH on GABA production, three batch processes with different pH controlled at 4.5, 5.0 and 5.5, respectively, were conducted. It was found that the highest GABA yield of 40.73 g·L^-1 can be obtained at 72 h with pH controlled at 5.0. The fed-batch fermentation of GABA was studied preliminarily; it was found that, after four times L-sodium glutamate （L-MSG） addition, the yield of GABA reaches 76.36 g·L^-1 at 108 h, and this GABA yield which is enhanced significantly by fed-batch fermentation is 128.6%, 219% and 87.5%, respectively, higher than that of shake flask fermentation, anaerobic fermentation and fermentation with pH controlled at 5.0.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2008年第4期618-623,共6页
Journal of Chemical Engineering of Chinese Universities
基金
国家自然科学基金(30570411)
国家“973”计划资助项目(2007CB714305)
浙江省重点科技计划项目
关键词
Γ-氨基丁酸
培养条件
PH控制
分批发酵
补料发酵
γ-aminobutyric acid
cultivation conditions
pH control
batch fermentation
fed-batch fermentation
