摘要
食品有害物质与DNA相互作用模式的体外研究方法,包括电化学法、紫外-可见吸收光谱法、荧光光谱法、凝胶电泳法和黏度法等;食品有害物质与DNA相互作用的定量方法,包括32P后标记法、免疫分析法、免疫毛细血管电泳-激光诱导荧光法和液相色谱-串联质谱法等。本文在查阅近10a相关文献的基础上,对体外研究DNA相互作用的检测技术和分析方法进行综述,以期为食品有害物质与DNA的相互作用研究提供一定的参考。
The research methods such as electrochemical, UV-visible, fluorescence, SCGE and viscosity methods for the interaction between harmful substances in foods and DNA have been reviewed. A number of techniques and methods such as 32P-postlabelling, immunoassay, immuno-CE/LIF and LC/MS have also been developed for the quantitative detection of interaction between harmful substances in foods and DNA. Based on the research of recently 10 years of related literature, the detection technique and analytic methods regarding in vitro investigation of DNA interaction have been also briefly discussed in this article, which will provide valuable references for the studies of interaction between food harmful substances and DNA.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第7期302-306,共5页
Food Science
基金
国家自然科学基金项目(31171654)
