期刊文献+

原料乳中产β-内酰胺酶菌株的快速筛选和鉴定 被引量:5

Rapid Isolation and Identification of β-Lactamase-Producing Strains in Raw Milk
下载PDF
导出
摘要 利用青霉素-藤黄微球菌-TSA培养基建立一种快速筛选原料乳中产β-内酰胺酶细菌的方法,该筛选方法比常规方法更加便捷,易于观察,并且具有很高的特异性分离鉴别能力,同时通过细菌16S rDNA序列比对方法来鉴定所分离的产酶菌的种属。结果表明,原料乳中存在产β-内酰胺酶的菌株,主要产酶菌为大肠杆菌。 Penicillin-Micrococcus luteus-TSA medium plates were used to establish a method for the rapid isolation of β-lactamase-producing bacteria in raw milk. This method revealed a more convenient and simpler observation than conventional methods, and could provide highly specific isolation. 16S rRNA gene sequence analysis allowed the assignment of the β-lactamase-producing bacterial isolates obtained in this study. As a result, Escherichia coli was the dominant β-lactamase-producing strain in raw milk.
出处 《食品科学》 EI CAS CSCD 北大核心 2013年第7期162-165,共4页 Food Science
基金 “十二五”国家科技支撑计划项目(2012BAK17B04) 国家“863”计划项目(2011AA100902) 国家自然科学基金项目(31171718) 黑龙江省教育厅科学技术研究重点项目(12511z005)
关键词 Β-内酰胺酶 原料乳 菌株分离 菌株鉴定 β-lactamase raw milk isolation identification
  • 相关文献

参考文献16

  • 1李伟华,袁仲,张慎举.兽药残留对动物性食品安全的影响与控制措施[J].安徽农业科学,2007,35(19):5864-5865. 被引量:30
  • 2HAMANN J,TOLLE A,HEESCHEN W,et al.Antibiotics and sulphonamides in milk and milk products[J].International Dairy Federation:Brussels,1979:43-56. 被引量:1
  • 3JACOBY G A.Beta-lactamase nomenclature[J].Antimicrobial Agents and Chemotherapy,2006,50:1123-1129. 被引量:1
  • 4BARRY G H.Evolution of the serine β-lactamases:past,present and future[J].Drug Resistance Updates,2004,7:111-123. 被引量:1
  • 5LI Xianzhi,MANISHA M,SHIVA G,et al.β-Lactam resistance and β-lactamases in bacteria of animal origin[J].Veterinary Microbiology,2007,121:197-214. 被引量:1
  • 6MAJA B,ANDREA M H,ROBERT A B,et al.What's new in antibiotic resistance? Focus on beta-lactamases[J].Drug Resistance Updates,2006,9:142-156. 被引量:1
  • 7SULLIVAN T J,WEDNER H J,SHATZ G S,et al.Skin testing to detect penicillin allergy[J].Journal of Allergy and Clinical Immunology,1981,68:171-180. 被引量:1
  • 8ZHE Xu,WANG Haoyang,HUANG Shixin,et al.Determination of β-lactamase residues in milk using matrix-assisted laser desorptiorff ionization fourier transform mass spectrometry[J].Analytical Chemistry,2010,82(5):2113-2118. 被引量:1
  • 9张秀芹,李欣南,田颖.生鲜牛乳中内源性β-内酰胺酶的研究[J].中国乳业,2009(11):58-59. 被引量:12
  • 10ALDO C,ASPAN A,ARTUSSON K,et al.Genotypic variation among Staphylococcus aureus isolates from cases of clinical mastitis in Swedish dairy cows[J].The Veterinary Journal,2010,8:188-192. 被引量:1

二级参考文献21

共引文献57

同被引文献36

引证文献5

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部