摘要
目的探讨β-葡聚糖对Wistax大鼠增生性玻璃体视网膜病变（PVR）的影响。方法52只Wistax大鼠随机分为空白对照组（A）、模型对照组（B）及β-葡聚糖高（C）、中（D）、低（E）浓度治疗组。A组4只，B组、C组、D组及E组每组为12只，以富含血小板血浆（PRP）注入B组、c组、D组及E组大鼠玻璃体腔内建立PVR模型，A组注入生理盐水。建模后B组向大鼠实验眼玻璃体内注入生理盐水；C组注入0．04g/m1的B．葡聚糖溶液；D组注入0．02g／ml的β-葡聚糖溶液；E组注入0．01g／ml的β-葡聚糖溶液。各组于制模后1．3，7，14，21，28d行裂隙灯及间接眼底镜检查，比较各组间PVR分级程度有无差异。结果术后间接眼底镜检查表明，第l—14天各组间PVR分级程度差异无统计学意义（P〉0．05）。β-葡聚糖高浓度治疗组PVR分级程度在术后第2l天及第28天均明显低于模型对照组（P〈0．05）；β-葡聚糖中浓度治疗组及β-葡聚糖低浓度治疗组PVR分级程度仅在术后第28天明显低于模型对照组（P〈0．05）。结论β-葡聚糖可能通过某种机制抑制PVR发展，具有防治PVR的潜能，
Objective To investigate the effect and anti-proliferative vitreoretinopathy mechanism of β-glucan to experimental Wistar rats with proliferative vitreoretinopathy. Methods Fifty-two wistar rats were randomly divided into control group（A） , model control gronp（B） ,β-glucan of high concentration treatment group（C） , β-glucan of medium concentration treatment group（D） and β-glucan of low concentration treatment group（E）. Group A had 4 rats,group B,C,D and E had 12 rats. Rats of group B,C,D and E were intravitreal injected platelel rich plasms to establish proliferative xitreoretinopathy rats model;rats of group A were intravitreal injected saline. After modeling rats of group B were intravitreal injected saline;rats of group C were intravitreal injected β-glucan solution of 0.04g/ml;group D O. 02g/ml; group E 0.01g/ml. All rats were taken slit lamp examination and indirect ophthalmoscopy on 1 day,3 days,7 days,14 days,21 days and 28 clays after modeling,the PVR grade level of every group were compared to see whether there were differences. Results The ,esuh of the indirect ophthahnoscopy showed that the PVR grade level was not statistically significant among ever）., group at 1 day,3 days,7 days, 14 days（ P 〉 0.05）. The PVR grade level of β-glucan of high concentration treatment group was significantly lower than the model control group at 21 clays and 28 days after ,molding{P 〈 0.05） ;the PVR grade level of β-glucan of medium concentration treatment group and low concentration treatment group was significantly lower than model control group only at 28 days after molding（ P 〈 0.05 ）. Conclusion β-glucan may inhibit the develupment of PVR through some mechanism. It may have the potential of prevention and treatment of PVR.
出处
《潍坊医学院学报》
2013年第1期32-34,共3页
Acta Academiae Medicinae Weifang
