摘要
目的研究盐生植物多枝柽柳中抑制血管紧张素Ⅰ转换酶(ACE)活性的成分。方法采用硅胶柱色谱、葡聚糖凝胶柱色谱等方法对多枝柽柳活性部位进行分离,运用现代光谱技术鉴定化合物结构。通过HPLC法测定各萃取物及酚类化合物对血管紧张素Ⅰ转换酶的抑制活性。结果从多枝柽柳中分离得到3种酚类化合物、2种甾醇类化合物,分别为异阿魏酸(1),4-羟基-3,5-二甲醚苯甲醛(2),4-羟基-3,5-二甲醚苯甲酸(3),β-谷甾醇(4),胡萝卜苷(5)。依据萃取物的活性情况,对3种酚类化合物进行了抑制血管紧张素Ⅰ转换酶活性的测定,在质量浓度为25mg.mL-1时,化合物1的抑制活性最高,抑制率可达82%,化合物2和3亦具有一定的抑制活性。结论化合物2和3为首次从该植物分离得到。依据活性追踪方法,化合物1,2和3均具有一定的抑制血管紧张素Ⅰ转换酶活性。
Objective To investigate the chemical constitutes of Tamaix ramosissima Ledeb.and the inhibitory activity on ACE.Methods The constitutes were isolated by chromatography on silica gel,Sephadex LH-20,and their structures were elucidated on the basis of spectral analysis.The inhibitory activity on ACE was evaluated by HPLC method.Results Five compounds were isolated and identified as isoferulic acid（1）,4-hydroxy-3,5-dimethoxy-benzaldehyde（2）,4-hydroxy-3,5-dimethoxy-benzic acid（3）,β-sitosterol（4）,daucosterol（5）.The inhibitory activity of compounds 1-3 on ACE was investigated.Compound 1 showed the highest activity at the concentration of 25 mg·mL-1,the inhibitory rate was 82%;The inhibitory activity of compound 2 showed dose-independence.The inhibitory rate of compound 3 was 55% at the concentration of 12.5 mg·mL-1.Conclusion Compounds 2 and 3 were isolated from Tamarix ramosissima Ledeb.for the first time.Compound 1,2,3 showed certain inhibitory activity on ACE.
出处
《西北药学杂志》
CAS
2013年第2期114-116,共3页
Northwest Pharmaceutical Journal
基金
新疆少数民族科技人才特殊培养科技项目(编号:201223111)
