摘要
【目的】探讨血管紧张素Ⅰ转换酶(ACE)基因插入与缺失(I/D)多态位点与糖尿病冠心病中医证候的关系。【方法】用聚合酶链反应(PCR)-2％琼脂凝胶电泳法,捡测了52名糖尿病冠心病患者人血ACE基因I/D多态性,根据中医辨证,将其分为血瘀和肾阳虚2个亚组,并与41名正常人作比较。【结果】糖尿病冠心病组DD型频率和D等位基因频率均高于正常组(分别P<0.01和P<0.05);在糖尿病冠心病中,血瘀组的ID型频率高于肾阳虚组(P<0.01),而DD型频率却低于肾阳虚组(P<0.05),2组 I、D等位基因频率分布未见显著性差异,并均以D等位基因占优势。【结论】ACE基因I/D多态性分布与糖尿病冠心病的发病和病情转归预后有关,D型等位基因可能是糖尿病冠心病,尤其是肾阳虚衰型发生的内在因素。
【Objective】To investigate the relationship between angiotensin Ⅰ-converting enzyme (ACE) gene insertion/
deletion (I/D) polymorphism and different syndrome patterns of diabetic coronary heart disease ( DCHC ) . 【Methods】
Fifty-two cases of DCHC (Group A) were differentiated as blood-stagnation (BS) pattern and kidney-yang deficiency
(KYD) pattern. ACE gene I/D polymorphism in DCHC patients was examined with polymerase chain reaction method and
2% agar gel electrophoresis and was compared with that in 41 healthy volunteers (Group B) .【Results】The frequency of
genotype DD and the allelic gene D in Group A was higher than that in Group B (P< 0.01 or P< 0.05) . In Group A, the
frequency of genotype ID was higher and that of genotype DD was lower in patients with blood-stasis pattern than those with
KYD pattern ( P< 0.05); the differences of allelic gene I and allelic gene D distribution frequency were insignificant be-
tween the two patterns and the allelic gene D was in predominance.【Conclusion】There is a certain relationship between
ACE gene I/D polymorphism and the occurrence and prognosis of DCHC. The dominant allelic gene D in genotypes may be
correlated with the occurrence of DCHC, especially with the kidney-deficiency pattern.
出处
《广州中医药大学学报》
CAS
2003年第4期261-263,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药管理局资助课题(编号:98332)
关键词
糖尿病
冠心病
中医证候
血管紧张素Ⅰ转换酶
基因多态性
聚合酶链反应
肾阳虚衰型
DIABETIC CORONARY HEART DISEASE/pathogenesis (TCD)
ANGIOTENSIN-CONVERTING ENZYME
GENE FREQUENCY
BLOOD STASIS
KIDNEY-YANG DEFICIENCY
