摘要
为了建立双孢蘑菇野生种质杂交育种技术平台,选育出具有适应性强的高产优质双孢蘑菇杂交品种,应用双孢蘑菇同核不育株间的杂交育种技术,进行国内外野生菌株间及野生菌株与栽培菌株间的配对杂交。结果表明:双孢蘑菇四孢变种菌株的同核不育株分离比率最大,达92.6%;传统栽培菌株分离的同核不育株分离比率较小,仅8.7%。单孢分离的同核不育株菌落形态存在着较大的差异,其中杂交菌株分离的同核不育株菌落形态类型变化较多,传统栽培菌株的变化较少。四川野生菌株分离的同核不育株与国外野生菌株及国内栽培菌株分离的同核不育株间均可杂交,进一步证实四川分离的野生菌株是我国青藏高原地区的双孢蘑菇野生种质。
To set up the technology platform for the crossbreeding of Agaricus bisporus wild strains,and screen out new hybrid cultivars with strong adaptability,high production and good quality,using the crossbreeding technology of Agaricus bisporus homokaryons,the hybridizations between domestic and foreign wild strains,wild strains and cultivation strains were carried out.The results showed that,the proportion of sterile homokaryons in Agaricus burnettii monospore isolates was the highest,up to 92.6%.It was the lowest in traditional cultivation strain,only 8.7%.The colony shape of homokaryons made much difference,and the homokaryons derived from hybrid strain diversified more than those from traditional strains.The homokaryons separated from Sichuan wild strains could be intercrossed with those from foreign wild strains or domestic cultivation strains,further indicating the wild strains from Sichuan to be Agaricus bisporus new germplasm in Tibet altiplano area of China.
出处
《中国农学通报》
CSCD
2013年第7期93-98,共6页
Chinese Agricultural Science Bulletin
基金
国家科技支撑计划课题"农牧废弃物作食用菌培养料的多级循环与增值利用技术研究与示范"(2012BAD14B15)
福建省农科院科技创新团队重点科研计划项目"双孢蘑菇野生种质创新育种技术平台研究与制种产业化示范"(2011)
福建省自然科学基金重点项目"中国野生双孢蘑菇种质资源的调查
鉴定与重要性状评价"(B0620004)
关键词
双孢蘑菇
野生菌株
四孢变种
配对杂交
Agaricus bisporus
wild strain
sterile strain
hybridization
