摘要
采用L16(45)正交分析方法,对双孢蘑菇原生质体制备工艺进行了优化.试验结果表明,最佳制备条件是取菌龄7 d的液体菌丝体,以2.5%溶壁酶+7%蜗牛酶为组合,0.6 mol/L NaCl作为渗透压稳定剂,在30℃条件下,酶解3 h,原生质体产率为2.34×108个/mL.本研究可为双孢蘑菇原生质体的融合及转基因研究提供理论依据.
The technology for preparing Agaricuz bisporus protoplast were optimized by using L16 （4^5） orthogonal method. The results showed that the highest production rate of the protoplast was 2.34 × 10^8/mL under the conditions that 7-day-old liquid mycelium in the enzyme solution of 2.5% lysowallzyme and 7% snailzyme digested for 3 hours at temperature of 30 ℃ with 0.6 mol/L NaCl as osmotic pressure stabilizer. Theoritical base for studying the protoplast fusion and transgenic of Agaricuz bisporus is provided.
出处
《天津师范大学学报（自然科学版）》
CAS
2006年第4期32-35,共4页
Journal of Tianjin Normal University：Natural Science Edition
基金
天津市农委资助项目(04020)
关键词
双孢蘑菇
原生质体
正交分析法
产率
Agaricuz bisporus
protoplast
orthogonal method
production rate
