摘要
背景:脑蛋白水解物注射液能缓解多种神经系统疾病的症状,但其作用机制并不明确。研究显示,脑蛋白水解物注射液在体内和体外实验中有促进神经发生的潜能。目的:观察脑蛋白水解物注射液对培养的神经胶质抗原2阳性神经祖细胞增殖和分化的影响。方法:取成年大鼠海马原代及传代培养神经胶质抗原2细胞,以免疫荧光染色法鉴定细胞性质,以乳酸脱氢酶分析法测定细胞活性,以原位缺口末端标记技术(即TUNEL法)观察细胞凋亡,BrdU掺入法鉴定新生细胞。结果与结论:脑蛋白水解物注射液干预显著减少TUNEL阳性细胞数,并明显增加神经胶质抗原2细胞数和神经胶质抗原2细胞中微管相关蛋白2a+2b、突触蛋白Ⅰ、γ-氨基丁酸和囊泡γ-氨基丁酸转运体表达水平。说明脑蛋白水解物注射液能抑制神经胶质抗原2细胞凋亡,促进神经胶质抗原2细胞增殖,并能促进神经胶质抗原2细胞向神经元(特别是γ-氨基丁酸能抑制性中间神经元)分化。
BACKGROUND： Cerebrolysin can relieve the symptoms of many nervous system diseases, but the precise mechanism remains poorly understood. Cerebrolysin has been shown to promote neurogensis by in vivo and in vitro experiments. OBJECTIVE： To investigate the effects of cerebrolysin on the proliferation and differentiation of cultured neuroglia antigen 2-positive neural progenitor cells. METHODS： Neuroglia antigen 2 cells from the hippocampus of the adult rats were primary cultured and sub-cultured. Cell characteristics were identified by immunofluorescence staining. Cell viability was determined by lactate dehydrogenase assay. Cell apoptoSis was detected by TUNEL method. The newly generated cells were identified by BrdU incorporation. RESULTS AND CONCLUSION： Cerebrolysin significantly decreased the number of TUNEL-positive cells and significantly increased the number of neuroglia antigen 2-positive cells and the expression level ofmicrotubule-associated protein 2a＋2b, synapsin I, y-aminobutyric acid and vesiculary-aminobutyric acid transporter in neuroglia antigen 2-positive cells. These findings suggest that cerebrolysin can inhibit neuroglia antigen 2-positive cell apoptosis, promote neuroglia antigen 2-positive cell proliferation and differentiation into neurons （especially GABAergic inhibitory interneurons）.
出处
《中国组织工程研究》
CAS
CSCD
2013年第6期1081-1088,共8页
Chinese Journal of Tissue Engineering Research
基金
河北省教育厅科学研究计划项目(2009005)
河北省卫生厅重点科技研究计划(20100085)
河北省自然科学基金(C2011206075)
新华区科学技术研究与发展计划~~
关键词
干细胞
干细胞培养与分化
脑蛋白水解物注射液
神经胶质抗原2
神经祖细胞
增殖
分化
乳酸脱氢酶
微管相关蛋白2a+2b
γ-氨基丁酸
囊泡
Γ-氨基丁酸转运体
省级基金
干细胞图片文章
stem cells
stem cell culture and differentiation
cerebrolysin
neuroglia antigen 2
neural progenitorcell
proliferation
differentiation
lactate dehydrogenase
lactate dehydrogenase
microtubule-associated protein2a＋2b
y-aminobutyric acid
vesiculary-aminobutyric acid transporter
provincial grants-supported paper
stem cellphotographs-containing paper
