摘要
目的通过研究脑蛋白水解物注射液(CL)对体外培养神经祖细胞(NPCs)增殖和分化的影响,探讨其改善阿尔茨海默病认知功能的机理。方法从成年大鼠脑不同区域原代及传代培养神经祖细胞,以乳酸脱氢酶(LDH)分析法测定细胞活性,以免疫荧光染色法鉴定细胞性质,以蛋白印迹转移(Western blot)法检测蛋白质表达变化。结果CL处置明显增加神经祖细胞数及MAP2a/b、SynapsinⅠ表达水平。结论CL促进神经祖细胞增殖和向神经元分化,可能是其改善阿尔茨海默病认知功能的机理之一。
Objective To explore the mechanism of Cerebrolysin（CL）improving the cognitive function in Alzheimer＇s disease by studing the effects of CL on proliferation and differentiation of neural progenitor cells（NPCs）in vitro.Methods NPCs primary culture was done from different regions of the adult rat brain.The characteristics of the cells were identified by immunofluorescence stain,the cell activities were determined by lactate dehydrogenase（LDH）assay,and the expression level of proteins were identified by western blot.Results CL increased the number and the expression level of MAP2a/b and Synapsin I of NPCs significantly.Conclusion CL promotes proliferation and neuronal differentiation of NPCs,it may be one of the mechanisms that how CL improves the cognitive function in Alzheimer＇s disease.
出处
《脑与神经疾病杂志》
2010年第2期89-92,共4页
Journal of Brain and Nervous Diseases
基金
日本文部科学省科学研究费补助金资助项目(18390324)
2009年河北省人事厅留学人员科技活动项目择优资助项目
