摘要
目的探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)增殖的作用。方法根据文献从成年大鼠海马原代及传代培养NG2细胞,以免疫荧光染色法鉴定细胞性质,以乳酸脱氢酶(LDH)分析法测定细胞活性,以原位缺口末端标记技术(即TUNEL法)观察细胞凋亡,5'-溴脱氧尿嘧啶核苷(BrdU)掺入法鉴定新生细胞。结果CL处置明显增加NG2细胞数,并显著减少TUNEL阳性细胞数,但对BrdU阳性细胞数无明显影响。结论CL能抑制NG2细胞凋亡,从而促进NG2细胞的增殖。
Objective To investigate the effects of Cerebrolysin（CL） on the proliferation of the NG2 proteoglycan-positive neural progenitor cells （NG2 cells）.Methods NG2 cell primary culture was done from adult rat hippocampus according to the reference.The characteristics of the cells were identified by immunofluorescence stain,the cell activities were determined by Lactate dehydrogenase （LDH） assay,apoptosis was detected by TUNEL method,and the new born cells were identified by BrdU.Results CL increased the number of the NG2 cells,and decreased the number of TUNEL positive cells significantly,but had little effect on the BrdU positive cells.Conclusion CL increases the proliferation of NG2 cells by inhibiting the apoptosis.
出处
《脑与神经疾病杂志》
2009年第6期417-419,共3页
Journal of Brain and Nervous Diseases
基金
日本文部科学省科学研究费补助金资助项目(18390324)
2009年河北省人事厅留学人员科技活动项目择优资助项目
