摘要
目的建立HPLC-ELSD法测定十味芪术通窍合剂中黄芪甲苷的含量。方法采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相∶乙腈—水(35∶65),流速1.0 mL.min-1,ELSD参数:雾化温度36℃,漂移管温度90℃,气体压力35 psi。结果黄芪甲苷进样量在3.08～24.60μg范围内呈良好线性关系(r=0.999 7),平均加样回收率为98.5%(n=9)。结论该法操作简便,重复性好,灵敏度高,可有效控制十味芪术通窍合剂中黄芪甲苷含量。
Objective To establish an HPLC-ELSD method for determination of astragal-oside Ⅳ in shiweiqishutongqiao mixture.Methods Kromasil C18（250 mm×4.6 mm,5 μm）Column was used.The mobile phase consisted of acetonitrile-water（35∶ 65）and the flow rate was 1.0 mL·min-1.The ELSD parameters were atomization temperature 36℃,drift tube temperature at 90℃,gas pressure 35psi.Results Astragaloside Ⅳ had a good linear relation in the range of 3.08~24.60 μg（r=0.999 7）.The average recovery（n=9）was 98.5%.Conclusion This method is rapid,sensitive,and accurate,which can be used for the quality control of shiweiqishutongqiao mixture.
出处
《安徽医药》
CAS
2013年第3期402-404,共3页
Anhui Medical and Pharmaceutical Journal
