摘要
[目的]优化暑热宁合剂中绿原酸的高效液相色谱法测定条件。[方法]色谱柱:Xterra188 Waters RPC18;检测器:Waters2998;工作站:Waters Empower;流动相:乙腈-0.4%磷酸(10∶90);流速:1 mL/min;检测波长:327 nm。[结果]该条件下可使暑热宁合剂中主要成分绿原酸达到较好的分离。[结论]高效液相色谱(HPLC)实现该样品的简便、准确、快速、经济实用的分析。
[Objective] To optimize the chromatographic conditions for determining Chlorogenic Acid in Shuren ing（Summer Heat Ning） mixture.[Methods] Xterra188 Waters RP C18（250 mm×4.6 mm,5 μm） column was used;Detecter was waters2998;Work station： Waters Empower;The motile phase： acetonitrile0.4% Phosphoric acid（10∶90）,The flow rate was 1 mL.per min,and the detection wavelength was set at 327nm.[Results] This condition could have a good separation for Chlorogenic Acid.[Conclusion] It was a simple,accurate,fast and inexpensive analysis for the adenosine phosphates.
出处
《天津中医药大学学报》
CAS
2011年第1期43-44,共2页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家"重大新药创制"科技重大专项项目(2010ZX09102-201)
