摘要
目的探讨宫颈鳞癌组织中MDM2、p53的表达及与高危型人乳头瘤病毒(HPV)感染的关系。方法选取实验组30例宫颈鳞癌组织及对照组20例正常宫颈组织,石蜡包埋后,采用免疫组化法检测组织中MDM2、p53蛋白的表达;采用杂交捕获二代实验(HC2)的方法检测实验组及对照组宫颈分泌物中高危型HPV的表达。结果 MDM2、p53在宫颈鳞癌组织中的表达阳性率分别为90%、86.7%,高危型HPV(HR-HPV)在宫颈鳞癌患者宫颈分泌物中的检出率为83.3%;p53在HR-HPV阳性的宫颈鳞癌患者中阳性表达率为92%,与对照组比较差异均有统计学意义(P<0.05);经Spearman相关分析显示,P53与高危型HPV在宫颈鳞癌中的表达呈正相关(r=0.822,P<0.05)。结论 MDM2、p53与宫颈鳞癌的发生有关;高危型HPV感染可能是p53由抑癌基因转变为癌基因的关键因素。
Objective To detect the expression of MDM2, p53 and HR - HPV in patients with cervical canc-er. Methods （ 1 ） The expressions of MDM2, p53 were observed by immunihistochemisty in the cervical cancer tissues of 30 cases with cervical squamous cancer and the normal cervial tissues of 20 cases as controls. （2）The expressions of HR - HPV were observed by HC2 in the cervical squamous cancer tissues and normal cervica 1 tissues. Results The positive expression of MDM2,p53 lied in nucleus and the positive rate were 90%and 86.7%. The positive rate of HR - HPV in cervical squamous cancer was 83.3%. There was significantly statistical difference in MDM2 and P53 between and cervical squamous cancer tissues and normal cervical tis-sues. Conclusion The expression of MDM2 and p53 may be a key role in occurrence of cervical squamous cancer.
出处
《宁夏医科大学学报》
2013年第1期26-28,F0004,共4页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ10151)
