摘要
目的:探讨E6-AP3种转录本在宫颈癌的表达及其临床意义。方法:免疫组织化学SP法检测30例宫颈癌组织及20例正常宫颈组织中E6-AP蛋白的表达,分析E6-AP的表达与宫颈癌病理分级之间的关系。同时用W estern blot检测E6-AP在正常宫颈脱落细胞和宫颈癌脱落细胞的表达水平。RT-PCR方法明确E6-AP 3种转录本在正常宫颈组织和宫颈癌组织中的表达。结果:RT-PCR结果显示E6-AP3种亚型在宫颈癌组织中均存在,并且表达高于正常子宫颈组织,其中Ⅱ型表达水平很低,以Ⅰ型和Ⅲ型为主。免疫组化和W estern B lot结果显示E6-AP在宫颈癌组织表达水平高于正常宫颈组织。结论:E6-AP表达升高可能与宫颈癌的发病机制相关。
Objective：To evaluate E6-AP three kinds of transcripts in cervical carcinoma and its clinical significance.Methods：E6-AP protein expression were detected by Immunohistochemistry SP method in 30 cases of cervical cancer tissues and 20 cases of normal cervical tissue and the relationship between E6-AP expression and the pathological grade of cervical cancer was also analyzed.E6-AP expression in the normal exfoliated cervical cells and exfoliated cervical cancer cells were measured by western blot.RT-PCR method was performed to determine three kinds of explicit E6-AP transcripts expression in cervical carcinoma.Results：Overexpression of E6-AP typeⅠand Ⅲ transcripts and low expression of Ⅱ-type transcripts were found in the tissue and the exfoliated cells of cervical carcinoma tissue.The expression levels of E6-AP in cervical tissue and cervical exfoliated epithelial samples of cervical carcinoma were higher than normal cervical tissue and exfoliated cells（P0.05）.Conclusion：E6-AP expression may be related to increased pathogenesis of cervical cancer.
出处
《现代妇产科进展》
CSCD
北大核心
2010年第10期725-727,共3页
Progress in Obstetrics and Gynecology
基金
安徽省自然科学基金资助项目(No:090413117)
安徽省高等学校省级自然科学研究资助项目(No:KJ2010B375)
关键词
宫颈肿瘤
E6相关蛋白
泛素
Cervical cancer
E6-associated protein
Ubiquitin
