摘要
目的:检测喉鳞状细胞癌细胞中p-STAT3及p53蛋白的表达,探讨它们的临床意义及其相关性。方法:采用免疫组织化学技术,检测p-STAT3及p53蛋白在60例喉鳞状细胞癌和32例取自喉全(或近全)切除者癌旁(>2.0cm)处正常喉黏膜组织中的表达,应用显微图像分析系统测定阳性表达的平均光密度值,并进行统计分析。结果:在喉鳞状细胞癌组织中存在p-STAT3及p53蛋白的高表达;p-STAT3和p53蛋白的表达与喉鳞状细胞癌的临床分期、淋巴结转移有关(P<0.05);在喉鳞状细胞癌组织中p-STAT3和p53蛋白的表达存在正相关关系,相关系数r=0.655 8(P<0.01)。结论:p-STAT3和p53蛋白的表达与喉鳞状细胞癌的发生、发展和转移等生物学行为有关,两者的表达呈正相关关系。
Objective：To investigate the expression and clinical siginificance of phosphated signal transducer and activator of transcription 3（p-STAT3） and p53 protein in laryngeal squamous cell carcinoma（LSCC）, and the relationship between the two genes. Method：Formalin-fixed and paraffin-embedded tissues,which came from 60 cases of LSCC and 32 samples of normal mucosas over 2.0 cm away from tumor margin in 32 patients with total or subtotal laryngectomy were detected for the expression of p-STAT3 and p53 protein by in SP immunohistochemistry. Micro-image analysis system was used to determine the optical density, and the result was analyzed statistically. Result：There is over expression of p-STAT3 and p53 protein in LSCC. The expression of p-STAT3 and p53 protein in LSCC was associated with clinical stage and lymph nodal metastases（P〈0.05）. There was a positive correlation between the expression of p-STAT3 and P53 protein. The correlation coefficient （r） was 0. 655 8 （P〈0.01）. Conclusion：The expression of p-STAT3 and p53 protein may play an important role in the tumorigenesis,metastases and poor prognosis of LSCC. There was a positive correlation between the over expression of p- STAT3 and p53 protein in LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2013年第18期991-994,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
浙江省自然科学基金资助项目(No:LY12H13004)
