摘要
目的研究甲基汞(methylmereury,MeHg)致大鼠脑皮质的氧化损伤,并探讨N-乙酰半胱氨酸(N-acetylcysteine,NAC)对甲基汞致大鼠脑皮质氧化损伤的拮抗作用。方法将32只清洁级健康Wistar大鼠按体重随机分为4组,分别为对照(生理盐水)组、低剂量(4μmol/kg)MeHg组、高剂量(12μmol/kg)MeHg组和NAC(1 mmol/kg)预处理+MeHg(12μmol/kg)组,每组8只,雌雄各半。NAC预处理+MeHg组皮下注射NAC溶液,对照组和低、高剂量MeHg组均皮下注射生理盐水;注射2h后,采用腹腔注射方式染毒MeHg,连续染毒4周,测定脑组织蛋白质巯基、羰基和丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、三磷酸腺苷(ATP)酶的活力。结果 MeHg染毒大鼠脑皮质中MDA、蛋白质羰基、8-OHdG的含量升高(P<0.05),蛋白质巯基的含量降低和SOD、GSH-Px、ATP酶的活力降低(P<0.05);与高剂量MeHg组比较,NAC预处理+MeHg组大鼠脑皮质中上述指标均得到显著改善(P<0.05)。结论 MeHg可导致大鼠脑皮质的氧化损伤;NAC对甲基汞所致大鼠脑皮质氧化损伤有一定的拮抗作用。
Objective To study the oxidative damage induced by methylmercury （MeHg） in rats cerebral cortex,and investigate the protective effect of N-acetylcysteine （NAC） on the oxidative damage. Methods A total of 32 clean health Wistar rats were randomly divided into four groups,which were control group,4,12 μmol/kg MeHg group, injected with physiological saline subcutaneously, and NAC pretreatment＋ MeHg group injected with 1 mmol/kg NAC. After 2 h,control group was given intraperitioneal injection of saline,and two MeHg groups were given intraperitioneal injection of 4,12 μmol/kg MeHg,and NAC pretreatment ＋MeHg group were given intraperitioneal injection of 12 μmol/kg MeHg. The doses were 5 ml/kg. Exposure to MeHg once a day, continuously intervention and exposure to MeHg were performed for consecutive four weeks. 24 h after the last exposure,the brain cortex were collected and the levels of malondialdehyde （MDA）, sulfydryl （-SH）, carbonyl （-C -O - ）, 8 -hydroxy deoxyguanosine （8 -OHdG） and the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, adenosine triphosphatase （ATPase） were determined. Results With the increase of mercury dose, the levels of MDA, carbonyl, 8-OHdG increased,sulfydryl decreased, the activities of SOD, GSH-Px, ATPase were reduced. Compared with high-dose mercury group, in NAC pretreatment group,the above parameters were improved. Conclusion Methylmercury can lead to oxidative damage in rats cerebral cortex, NAC has certain antagonism on the oxidative damage-induced by methylmercury.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第3期193-197,F0003,共6页
Journal of Environment and Health
基金
国家自然科学基金(81172631)
