摘要
氧化还原信号转导,尤其是蛋白质巯基的氧化性修饰,与人类许多重要疾病关系密切,是目前国际上热点研究领域之一。然而长期以来,蛋白质巯基及其氧化修饰的专一、快速检测手段缺乏,尤其是在活细胞内源性检测方面几乎是空白,限制了该领域的发展。本文就近年来用于检测蛋白质巯基及其氧化修饰的化学方法作简要综述,并就基于巯基修饰发展荧光分子探针及其用于蛋白质组学研究,氧化还原相关疾病早期诊断作一展望。
Redox signal transduction,especially the oxidative modification of proein thiols,correlates with many diseases and becomes an expanding research area.However,there was rare method for quick and specific detection of protein thiols and their oxidative modification in living cells.In this article,we review the current chemical strategies for the detection and quantification of protein thiols and related cysteine oxidation.We also look into the future of the development of fluorescent probes for protein thiols and their potential application in the research of reactive cysteine proteomes and early detection of redox-related diseases.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第3期280-290,258,共11页
Acta Pharmaceutica Sinica
基金
国家重点基础研究发展计划(973计划)资助项目(2010CB126100)
国家高技术发展计划(863计划)资助项目(2011AA10A207)
国家自然科学基金资助项目(21076077)
