摘要
目的研究白藜芦醇(Res)在H2O2所致乳鼠心肌细胞损伤中的保护机制。方法将原代培养的乳鼠心肌细胞分成3组:对照组,H2O2处理组和Res+H2O2处理组,采用MTT比色法检测细胞活性,应用Real Time-PCR技术检测miR-34a的表达水平。结果 H2O2对乳鼠心肌细胞具有损伤作用,Res可明显缓解H2O2对乳鼠心肌细胞的损伤作用,经过H2O2处理后乳鼠心肌细胞内miR-34a的表达水平显著升高,Res可明显降低乳鼠心肌细胞内miR-34a的表达水平。结论 Res通过下调miR-34a的表达水平来发挥对H2O2所致乳鼠心肌细胞损伤的保护作用。
Objective To investigate the protective mechanism of resveratrol （Res） inH2O2induced neonatal cardiomyocytes injury. Methods Primary cultured neonatal cardiomyocytes were divided into 3 groups： control, H2O2 group and Res group. MTF assay was used to detect cell viability and real time PCR was used to detect miR-34a expression in each group. Results Res rescued neonatal cardiomyocytes injury caused by H2O2. Moreover, Res up-regulated the decreased miR-34a level induced by H2O2. Conclusion Res can rescue HaO2 induced neonatal cardiomyocytes injury by up-regulating miR-34a expression.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2013年第1期43-45,共3页
Journal of Harbin Medical University
基金
2010年黑龙江省教育厅科学技术研究面上项目(11551276)
